Skoufos Ioannis, Nelli Aikaterini, Venardou Brigkita, Lagkouvardos Ilias, Giannenas Ilias, Magklaras Georgios, Zacharis Christos, Jin Lizhi, Wang Jin, Gouva Evangelia, Skoufos Stylianos, Bonos Eleftherios, Tzora Athina
Laboratory of Animal Science, Nutrition and Biotechnology, Department of Agriculture, School of Agriculture, University of Ioannina, Kostakioi Artas, 47100 Arta, Greece.
Laboratory of Animal Health, Hygiene and Food Quality, Department of Agriculture, School of Agriculture, University of Ioannina, Kostakioi Artas, 47100 Arta, Greece.
Microorganisms. 2023 Jun 30;11(7):1723. doi: 10.3390/microorganisms11071723.
The aim of this study was to evaluate whether dietary supplementation with an innovative silage (IS) created using 60% olive mill waste, 20% grape pomace, and 20% deproteinised feta cheese waste solids can modulate the composition of the intestinal microbiota in weaned (Exp. 1) and finishing (Exp. 2) pigs. In Exp. 1 (40 day supplementation), forty-five crossbred weaned pigs were randomly assigned to the 0% (Control), 5%, or 10% IS groups (15 replicates/experimental diet). In Exp. 2 (60 day supplementation), eighteen finishing pigs from Exp. 1 were fed the control diet for 8 weeks before being re-assigned to their original experimental groups and fed with the 0% (Control), 5%, or 10% IS diets (six replicates/experimental diet). Performance parameters were recorded. Ileal and caecal digesta and mucosa were collected at the end of each experiment for microbiota analysis using 16S rRNA gene sequencing (five pigs/experimental diet for Exp. 1 and six pigs/experimental diet for Exp. 2). No significant effects on pig growth parameters were observed in both experiments. In Exp. 1, 5% IS supplementation increased the relative abundance of the family, genus, and (OTU_48) and reduced the relative abundance of genus in the caecum compared to the control and/or 10% IS diets ( < 0.05). In Exp. 2, 5% IS supplementation led to compositionally more diverse and different ileal and caecal microbiota compared to the control group ( < 0.05; = 0.066 for β-diversity in ileum). Supplementation with the 5% IS increased the relative abundance of // (OTU_3) in the ileum and caecum and (OTU_17) in the caecum and reduced the relative abundance of / (OTU_2) in the caecum compared to the control diet ( < 0.05). Similar effects on // and / were observed with the 10% IS diet in the caecum ( < 0.05). IS has the potential to beneficially alter the composition of the gastrointestinal microbiota in pigs.
本研究的目的是评估用60%的橄榄渣、20%的葡萄皮渣和20%的脱蛋白羊乳酪废渣固体物制成的创新青贮饲料（IS）进行日粮补充，是否能调节断奶仔猪（实验1）和育肥猪（实验2）肠道微生物群的组成。在实验1（40天补充期）中，45头杂交断奶仔猪被随机分配到0%（对照组）、5%或10%IS组（每组15个重复/实验日粮）。在实验2（60天补充期）中，实验1中的18头育肥猪先饲喂对照日粮8周，然后重新分配到原来的实验组，分别饲喂0%（对照组）、5%或10%IS日粮（每组6个重复/实验日粮）。记录生产性能参数。在每个实验结束时收集回肠和盲肠内容物及黏膜，用于通过16S rRNA基因测序进行微生物群分析（实验1每组5头猪，实验2每组6头猪）。在两个实验中均未观察到对猪生长参数的显著影响。在实验1中，与对照组和/或10%IS日粮相比，补充5%IS可增加盲肠中某科、某属和某操作分类单元（OTU_48）的相对丰度，并降低某属的相对丰度（P<0.05）。在实验2中，与对照组相比，补充5%IS导致回肠和盲肠微生物群在组成上更加多样化且不同（P<0.05；回肠β多样性P = 0.066）。与对照日粮相比，补充5%IS可增加回肠和盲肠中某菌属/某菌属（OTU_3）以及盲肠中某菌属（OTU_17）的相对丰度，并降低盲肠中某菌属/某菌属（OTU_2）的相对丰度（P<0.05）。在盲肠中，10%IS日粮对某菌属/某菌属也有类似影响（P<0.05）。IS有潜力有益地改变猪胃肠道微生物群的组成。
