Hain-Saunders Natasza M R, Knight Daniel R, Bruce Mieghan, Byrne David, Riley Thomas V
Centre for Biosecurity, and One Health, Harry Butler Institute, Murdoch University, Murdoch, WA 6150, Australia.
School of Biomedical Sciences, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, WA 6009, Australia.
Microorganisms. 2023 Jul 3;11(7):1743. doi: 10.3390/microorganisms11071743.
poses an ongoing threat as a cause of gastrointestinal disease in humans and animals. Traditionally considered a human healthcare-related disease, increases in community-associated infection (CDI) and growing evidence of inter-species transmission suggest a wider perspective is required for CDI control. In horses, is a major cause of diarrhoea and life-threatening colitis. This study aimed to better understand the epidemiology of CDI in Australian horses and provide insights into the relationships between horse, human and environmental strains. A total of 752 faecal samples from 387 Western Australian horses were collected. was isolated from 104 (30.9%) horses without gastrointestinal signs and 19 (37.8%) with gastrointestinal signs. Of these, 68 (55.3%) harboured one or more toxigenic strains, including PCR ribotypes (RTs) 012 ( = 14), 014/020 ( = 10) and 087 ( = 7), all prominent in human infection. Whole-genome analysis of 45 strains identified a phylogenetic cluster of 10 closely related RT 012 strains of equine, human and environmental origin (0-62 SNP differences; average 23), indicating recent shared ancestry. Evidence of possible clonal inter-species transmission or common-source exposure was identified for a subgroup of three horse and one human isolates, highlighting the need for a One Health approach to surveillance.
作为人类和动物胃肠道疾病的病因，它构成了持续的威胁。传统上被认为是一种与人类医疗保健相关的疾病，社区获得性感染（CDI）的增加以及种间传播的证据不断增多，这表明控制CDI需要更广阔的视角。在马匹中，它是腹泻和危及生命的结肠炎的主要原因。本研究旨在更好地了解澳大利亚马匹中CDI的流行病学，并深入了解马、人和环境菌株之间的关系。共收集了来自387匹西澳大利亚马的752份粪便样本。在无胃肠道症状的104匹（30.9%）马和有胃肠道症状的19匹（37.8%）马中分离出了该病菌。其中，68株（55.3%）携带一种或多种产毒菌株，包括PCR核糖体分型（RTs）012（n = 14）、014/020（n = 10）和087（n = 7），这些在人类感染中都很常见。对45株菌株的全基因组分析确定了一个由10株密切相关的RT 012菌株组成的系统发育簇，这些菌株来自马、人和环境（单核苷酸多态性差异为0 - 62；平均为23），表明它们最近有共同的祖先。在一组三匹马和一株人类分离株中发现了可能的克隆种间传播或共同来源暴露的证据，这突出了采用“同一健康”方法进行监测的必要性。
