Biosecurity and One Health Research Center, Harry Butler Institute, Murdoch University, Western Australia, Australia.
School of Biomedical Sciences, The University of Western Australia, Western Australia, Australia.
Appl Environ Microbiol. 2023 Oct 31;89(10):e0037923. doi: 10.1128/aem.00379-23. Epub 2023 Oct 12.
is a leading cause of infectious diarrhea in humans and production animals and can be found in a variety of environmental sources. The prevalence and diversity of multi-locus sequence type clade 5 strains of in Australian production animals suggest Australia might be the ancestral home of this lineage of One Health importance. To better understand the role of the environment in the colonization of humans and animals in Australia, it is important to investigate these endemic sources. This study describes the prevalence, molecular epidemiology, and biogeographic distribution of in soils of Western Australia. A total of 321 soil samples from remote geographical locations across the eight health regions of Western Australia were screened for and isolates characterized by PCR ribotyping and toxin gene profiling. was isolated from 31.15% of samples, with the highest prevalence in the Perth Metropolitan Health Region (49.25%, = 33/67). Overall, 52 different strains [PCR ribotypes (RTs)] were identified, with 14 being novel, and 38% (38/100) of isolates being toxigenic, the most common of which was RT014/020. Five unique novel isolates showed characteristics similar to clade 5. This is the first study of isolated from soils in Australia. The high prevalence and heterogeneity of strains recovered suggest that soils play a role in the survival and environmental dissemination of this organism, and potentially its transmission among native wildlife and production animals, and in community and hospital settings.IMPORTANCE is a pathogen of One Health importance. To better understand the role of the environment in human and animal colonization/infection, it is critical that autochthonous reservoirs/sources of be investigated. This is the first study of isolated from soils of Western Australia (WA). Here, the ecology of in WA is described by examining the geographic distribution, molecular epidemiology, and diversity of isolated from soils across WA.
是人类和生产动物感染性腹泻的主要原因，可在多种环境来源中发现。澳大利亚生产动物中多基因序列型 5 群菌株的流行率和多样性表明，澳大利亚可能是该具有重要意义的“同一健康”谱系的起源地。为了更好地了解环境在澳大利亚人类和动物定殖中的作用，调查这些地方性来源非常重要。本研究描述了西澳大利亚土壤中 的流行率、分子流行病学和生物地理分布。从西澳大利亚 8 个卫生区偏远地理位置采集的 321 个土壤样本进行了 和 PCR 核糖体分型和毒素基因谱特征鉴定的分离株筛选。从 31.15%的样本中分离出 ，珀斯大都市区卫生区的患病率最高（49.25%，33/67）。总体而言，鉴定出 52 种不同的菌株[PCR 核糖体型（RT）]，其中 14 种为新型菌株，38%（38/100）的分离株为产毒型，最常见的是 RT014/020。五种独特的新型分离株表现出与 5 群相似的特征。这是首次在澳大利亚土壤中分离出 。从回收的 菌株的高流行率和异质性表明，土壤在该生物体的生存和环境传播中发挥作用，并且可能在当地野生动物和生产动物之间，以及在社区和医院环境中传播。
是一种具有重要意义的人类与动物共患病原体。为了更好地了解环境在人类和动物定殖/感染中的作用，必须对 的本地储库/来源进行调查。这是首次从西澳大利亚州（WA）的土壤中分离出 。在这里，通过检查 WA 土壤中 的地理分布、分子流行病学和多样性，描述了 WA 中 的生态学。
