Kartsev Nikolay N, Detusheva Elena V, Kalmantaeva Olga V, Korobova Olga V, Gerasimov Vladimir N, Kombarova Tatiana I, Borzilov Aleksander I, Fursova Nadezhda K, Vereshchagin Anatoly N, Svetoch Edward A
Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory "Kvartal A", 142279 Obolensk, Russia.
Laboratory of Molecular Biology, State Research Center for Applied Microbiology and Biotechnology, Territory "Kvartal A", 142279 Obolensk, Russia.
Microorganisms. 2023 Jul 7;11(7):1771. doi: 10.3390/microorganisms11071771.
In the last decade, the importance of hetero-pathogenic enteroaggregative Shiga-toxin-producing for public health has increased. Recently, we described the genetic background of the EAHEC O181:H4 strain of ST678 carrying the gene in prophage and five plasmids, including the plasmid-carrying and genes. Here, we present the morphological and enzymatic characteristics of this strain, as well as susceptibility to antimicrobials, biofilm formation, etc. Methods: Bacterial morphology was studied using an electron microscope. Susceptibility to antimicrobials was determined using the microdilution method. Cytotoxicity was estimated in Vero cells. Virulence was studied on mice.
The morphological and enzymatic properties of the hetero-pathogenic EAHEC strain were typical for ; electron microscopy revealed the specific flagella. The strain was susceptible to most antibiotics and disinfectants but resistant to ampicillin and ciprofloxacin and showed a high degree of biofilm formation. Cytotoxicity towards Vero cells was estimated as 80%.
The emergence of a new O181:H4 EAHEC strain poses a potential threat to humans because of the virulence potential that must be taken into account in the epidemiological analysis of outbreaks and sporadic cases of foodborne infections associated with hemolytic-uremic syndrome.
在过去十年中，异源性产志贺毒素的聚集性肠杆菌对公共卫生的重要性日益增加。最近，我们描述了携带噬菌体和五个质粒（包括携带stx和eae基因的质粒）的ST678型EAHEC O181:H4菌株的遗传背景。在此，我们展示该菌株的形态学和酶学特征，以及其对抗菌药物的敏感性、生物膜形成等情况。方法：使用电子显微镜研究细菌形态。采用微量稀释法测定对抗菌药物的敏感性。在Vero细胞中评估细胞毒性。在小鼠身上研究毒力。
异源性EAHEC菌株的形态学和酶学特性对于肠聚集性大肠杆菌是典型的；电子显微镜显示出特定的鞭毛。该菌株对大多数抗生素和消毒剂敏感，但对氨苄西林和环丙沙星耐药，并表现出高度的生物膜形成能力。对Vero细胞的细胞毒性估计为80%。
新的O181:H4 EAHEC菌株的出现对人类构成潜在威胁，因为其毒力潜力在与溶血尿毒综合征相关的食源性感染暴发和散发病例的流行病学分析中必须予以考虑。
