Setyarini Wahyu, Raharjo Dadik, Arizandy Radita Yuniar, Pamoengkas Zakaria, Sudarmo Subijanto Marto, Athiyyah Alpha Fardah, Shirakawa Toshiro
Gastroenteritis Study Group, Institute of Tropical Disease.
Department of Pediatrics, Faculty of Medicine.
Infect Dis Rep. 2020 Jul 7;12(Suppl 1):8745. doi: 10.4081/idr.2020.8745.
Enteroaggregative haemorrhagic (, EAHEC) has been identified as the agent responsible for one of the largest outbreaks of gastroenteritis and Haemolytic-uremic syndrome (HUS) that is transmitted through food in Germany in 2011. The hypervirulent pathotype has a unique combination of two pathogens namely enterohemorrhagic strain (EHEC) which produces shiga/verotoxin and enteroaggregative toxins (EAEC) which produces toxins similar to ST and hemolysin. The toxin produced by the EAHEC strain is a hybrid pathotype that combines the virulence potential of the EAEC and EHEC strains that will damage the microcirculation, cause vasculitis and other toxic effects. The purpose of this study was to determine the percentage of samples infected with enteroaggregative hemorrhagic bacteria (EAHEC) in pediatric diarrhea patients at DR. Soetomo Hospital, Surabaya, Indonesia, 2015 This study used PCR (Polymerase Chain Reaction) method to detect enteroaggregative strains (CVD432 and aaic genes) and enterohemorrhagic (eae gene).The results showed that 33 out of 40 (82,5%) stool samples examined were detected enteroaggregative (EAEC), 4 out of 40 (10%) enterohemorrhagic (EHEC) and 3 out of 40 (7,5%) enteroaggregative haemorrhagic bacteria (EAHEC), which caused diarrhea in pediatric diarrhea patients at Dr. Soetomo General Hospital. The unique combination of genomic features of the Surabaya outbreak strain, containing characteristics from pathotypes EAEC and EHEC, suggested that it represents a new pathotype enteroaggregative haemorrhagic (EAHEC). It is expected that development of specific primer design and sequencing are needed to continue in this research.
肠聚集性出血性大肠杆菌（EAHEC）已被确定为2011年德国通过食物传播的最大规模肠胃炎和溶血尿毒综合征（HUS）疫情之一的病原体。高毒力致病型具有两种病原体的独特组合，即产生志贺毒素/维罗毒素的肠出血性大肠杆菌菌株（EHEC）和产生类似于耐热肠毒素和溶血素毒素的肠聚集性大肠杆菌毒素（EAEC）。EAHEC菌株产生的毒素是一种混合致病型，结合了EAEC和EHEC菌株的毒力潜能，会损害微循环、导致血管炎和其他毒性作用。本研究的目的是确定2015年印度尼西亚泗水苏托莫医院儿科腹泻患者中感染肠聚集性出血性细菌（EAHEC）的样本百分比。本研究采用聚合酶链反应（PCR）方法检测肠聚集性大肠杆菌菌株（CVD432和aaic基因）和肠出血性大肠杆菌（eae基因）。结果显示，在40份粪便样本中，有33份（82.5%）检测出肠聚集性大肠杆菌（EAEC），4份（10%）检测出肠出血性大肠杆菌（EHEC），3份（7.5%）检测出肠聚集性出血性大肠杆菌（EAHEC），这些细菌导致了苏托莫综合医院儿科腹泻患者的腹泻。泗水疫情菌株基因组特征的独特组合，包含了EAEC和EHEC致病型的特征，表明它代表了一种新的致病型肠聚集性出血性大肠杆菌（EAHEC）。预计本研究需要继续进行特异性引物设计和测序的开发。
