Zeng Yuhao, Chen Yu, Duan Chengchen, Jiang Xuelian, Wang Yufei, Zhang Linglin
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610017, China.
Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610017, China.
Microorganisms. 2023 Jul 13;11(7):1796. doi: 10.3390/microorganisms11071796.
The acidogenicity of is important for caries development. The antimicrobial peptide GH12 can affect the integrity of cellular membranes and the virulence factors of . Combining GH12 and NaF (GF) efficiently controlled the development of caries, but its mechanisms remained unrevealed. This research intended to verify the effects of GF on the acidogenicity of biofilms and to reveal the mechanisms. Lactic acid production assays and pH monitoring assays were conducted to investigate the regulatory effects of the GF treatment on the acidogenicity of biofilms. RNA sequencing and bioinformatics analyses were conducted to screen the transcriptional profile affected by the GF treatment. The results demonstrated the GF group had significantly less lactic acid and maintained the broth's pH values above 5.0 for longer times. Thereafter, GO/KEGG enrichment analyses and RT-qPCR validation revealed that the GF treatment mainly restrained the expression of genes related to the carbohydrates' internalization and metabolism. Compared with NaF, the GF treatment further downregulated the carbohydrates transportation genes. Moreover, compared with GH12, the GF treatment affected the membrane's integrity more significantly. Generally, GF treatment could arrest the acidogenicity of biofilms, mainly through suppressing carbohydrates transportation and inhibiting overall metabolism.
[微生物名称]的产酸性对龋齿发展很重要。抗菌肽GH12可影响细胞膜完整性及[微生物名称]的毒力因子。联合使用GH12和NaF（GF）可有效控制龋齿发展，但其机制仍未明确。本研究旨在验证GF对[微生物名称]生物膜产酸性的影响并揭示其机制。进行乳酸生成测定和pH监测测定以研究GF处理对[微生物名称]生物膜产酸性的调节作用。进行RNA测序和生物信息学分析以筛选受GF处理影响的转录谱。结果表明，GF组乳酸显著减少，且肉汤pH值在5.0以上维持较长时间。此后，GO/KEGG富集分析和RT-qPCR验证表明，GF处理主要抑制与碳水化合物内化和代谢相关基因的表达。与NaF相比，GF处理进一步下调碳水化合物转运基因。此外，与GH12相比，GF处理对膜完整性的影响更显著。总体而言，GF处理可通过抑制碳水化合物转运和抑制整体代谢来阻止[微生物名称]生物膜的产酸性。
