Department of Pharmacy and Nutrition, Center for Exact, Natural and Health Sciences, Federal University of Espirito Santo, Alto Universitário, S/N Guararema, Alegre 29500-000, ES, Brazil.
Nutrients. 2023 Jul 24;15(14):3281. doi: 10.3390/nu15143281.
Colorectal cancer has a high worldwide incidence. The aim of this study was to determine the effect of yacon flour (YF) on oxidative stress, inflammation, and endotoxemia in rats with induced colorectal cancer (CRC). The Wistar male rats were divided and kept for 8 weeks in four groups: S (basal diet, = 10), Y (YF flour + basal diet, = 10), C (CRC-induced control + basal diet, = 12), CY (CRC-induced animals + YF, = 12). CRC was induced by intraperitoneal injections of 1,2-dimethylhydrazine (25 mg/kg body weight). Groups Y and CY received 7.5% of the prebiotic FOS from YF. The treatment with YF increased fecal secretory immunoglobulin A levels and decreased lipopolysaccharides, tumor necrosis factor alpha and interleukin-12. However, no effect was observed on the oxidative stress by the total antioxidant capacity of plasma, anion superoxide, and nitric oxide analysis of the animals ( < 0.05). The short-chain fatty acids acetate, propionate, and butyrate showed interactions with NF-κB, TLR4, iNOS, and NADPH oxidase by in silico analysis and had a correlation (by the Person analysis) with CRC markers. The yacon flour treatment reduced the inflammation in rats with induced CRC, and could be a promising food to reduce the damages caused by colorectal cancer.
结直肠癌在全球范围内发病率较高。本研究旨在探讨雪莲果粉（YF）对诱导结直肠癌（CRC）大鼠氧化应激、炎症和内毒素血症的影响。雄性 Wistar 大鼠被分为四组，分别饲养 8 周：S 组（基础饮食，n = 10）、Y 组（YF 粉+基础饮食，n = 10）、C 组（CRC 诱导对照+基础饮食，n = 12）、CY 组（CRC 诱导动物+YF，n = 12）。CRC 通过腹腔注射 1,2-二甲基肼（25 mg/kg 体重）诱导。Y 组和 CY 组给予 YF 中 7.5%的益生元 FOS。YF 治疗增加了粪便分泌型免疫球蛋白 A 水平，并降低了脂多糖、肿瘤坏死因子-α和白细胞介素-12。然而，动物血浆总抗氧化能力、阴离子超氧化物和一氧化氮分析并未观察到氧化应激的影响（<0.05）。通过计算机分析，短链脂肪酸乙酸盐、丙酸盐和丁酸盐与 NF-κB、TLR4、iNOS 和 NADPH 氧化酶相互作用，并与 CRC 标志物呈相关性（通过 Person 分析）。雪莲果粉治疗可减轻诱导 CRC 大鼠的炎症，有望成为一种减少结直肠癌损伤的食品。
