Kim Jae Young, Lee Ji-Hye, Yang Jong Min, Lee Seo-Yong, Park Sun Young, Jin Jong Sook, Kim Dohyun, Park Jung-Won, Park Jong-Hyeon, Park Sang Hyun, Ko Young-Joon
Animal and Plant Quarantine Agency, Gimcheon-si 39660, Republic of Korea.
Department of Research and Development, FVC, Gimcheon-si 39660, Republic of Korea.
Vaccines (Basel). 2023 Jun 26;11(7):1156. doi: 10.3390/vaccines11071156.
Foot-and-mouth disease (FMD) is a highly infectious disease affecting cloven-hoofed animals and causes significant economic losses to the livestock industry. The Type O PanAsia-2 (O PA-2) vaccine strain is protective against a wide range of serotype O FMD virus (FMDV) strains in East Asia, and A22 Iraq/24/64 (A22 IRQ) is the most widely used vaccine strain in FMD vaccine antigen banks. The aim of this study was to produce antigens from O PA-2 and A22 IRQ viruses using a 100 L bioreactor and evaluate the protective efficacy of varying antigen concentrations in pigs. More than 2 μg/mL of the antigen was recovered from the O PA-2 and A22 IRQ virus-infected supernatants. Further, inactivation of O PA-2 and A22 IRQ by binary ethyleneimine revealed that the viral titers decreased below 10 TCID/mL within 13 h and 9 h, respectively. The O PA-2 and A22 IRQ vaccines, containing 10 μg and 5 μg of antigen, respectively, provided protection against homologous viruses in pigs. This is the first report demonstrating that the antigens obtained from the pilot-scale production of O PA-2 and A22 IRQ are viable candidate vaccines. These results will pave the way for industrial-scale FMD vaccine production in South Korea.
口蹄疫（FMD）是一种影响偶蹄动物的高度传染性疾病，会给畜牧业造成重大经济损失。O型泛亚-2（O PA-2）疫苗株可抵御东亚地区多种O型口蹄疫病毒（FMDV）毒株，而A22伊拉克/24/64（A22 IRQ）是FMD疫苗抗原库中使用最广泛的疫苗株。本研究的目的是使用100 L生物反应器生产O PA-2和A22 IRQ病毒的抗原，并评估不同抗原浓度对猪的保护效果。从感染O PA-2和A22 IRQ病毒的上清液中回收的抗原浓度超过2μg/mL。此外，用双乙烯亚胺对O PA-2和A22 IRQ进行灭活处理后发现，病毒滴度分别在13小时和9小时内降至10 TCID/mL以下。分别含有10μg和5μg抗原的O PA-2和A22 IRQ疫苗对猪的同源病毒具有保护作用。这是第一份证明从O PA-2和A22 IRQ中试规模生产获得的抗原是可行候选疫苗的报告。这些结果将为韩国口蹄疫疫苗的工业化生产铺平道路。
