Animal and Plant Quarantine Agency, 175 Anyang-ro, Manangu, Anyang city 430-757, Gyeonggi-do, Republic of Korea.
Animal and Plant Quarantine Agency, 175 Anyang-ro, Manangu, Anyang city 430-757, Gyeonggi-do, Republic of Korea; Clinical Research Center of the Affiliated Hospital of Guangdong Medical College, No. 57 South Renmin Avenue, Zhanjiang 524001, China.
Vaccine. 2014 Apr 1;32(16):1882-9. doi: 10.1016/j.vaccine.2014.01.067. Epub 2014 Feb 10.
Foot-and-mouth disease (FMD) is a highly contagious infectious disease, and the use of vaccines is known to be effective for its prevention. In 2010/2011, there was an epidemic of the South East Asia (SEA) topotype in East Asian countries. We adapted the SEA topotype virus isolated in November 2010 in Korea in cells to analyze the characteristics of the virus and evaluate its possibility as a vaccine. After cell culture adaptation, the FMD virus particle 146S was purified to develop an inactivated oil vaccine for SEA or other topotypes. To measure its immunogenicity, pigs were inoculated with the experimental vaccine at different concentrations of the antigen. The results indicated that the groups immunized with at least 7.5 μg antigen were protected from homologous challenge. The immunized pigs were also protected against heterologous virus (ME-SA topotype) challenge. The genetic variations between the two field isolates and the adapted vaccine strains were identified in six amino acids by complete genome sequencing.
口蹄疫（FMD）是一种高度传染性的传染病，疫苗的使用被证明对其预防有效。2010/2011 年，东亚国家发生了东南亚洲（SEA）亚型的疫情。我们适应了 2010 年 11 月在韩国分离的 SEA 亚型病毒在细胞中的特性，以分析病毒的特性，并评估其作为疫苗的可能性。经过细胞培养适应后，纯化了口蹄疫病毒 146S 颗粒，以开发针对 SEA 或其他亚型的灭活油疫苗。为了测量其免疫原性，用不同浓度的抗原对猪进行了实验性疫苗接种。结果表明，用至少 7.5μg 抗原免疫的猪群可免受同源性挑战的保护。免疫猪也可免受异源病毒（ME-SA 亚型）的挑战。通过全基因组测序，在两个田间分离株和适应的疫苗株之间鉴定了六个氨基酸的遗传变异。
