用于检测重症肌无力中可提取肌肉抗原抗体的酶联免疫吸附测定。

Enzyme-linked immunosorbent assay for detection of antibodies to extractable muscle antigens in myasthenia gravis.

作者信息

Viana V S, Bueno C, Marchiori P E, Oliveira R M, Assis J L, Scaff M, Cossermelli W

出版信息

Acta Neurol Scand. 1986 Jun;73(6):599-601. doi: 10.1111/j.1600-0404.1986.tb04606.x.

DOI:10.1111/j.1600-0404.1986.tb04606.x

Abstract

A purified citric acid soluble extract from human skeletal muscle (AEMA) and a phosphate-buffered saline extract from rabbit muscle acetone powder (EMA) were used to coat polystyrene beads in an enzyme-linked immunosorbent assay (ELISA). From 54 patients with myasthenia gravis, positive results were observed in 14. Five of 6 patients with thymoma had high levels of antibodies. With the diagnostic difficulties in detecting small and medium-sized thymoma, a sensitive assay for detection of antibodies to muscle antigen may be an important supplementary tool to detect tumors at early stages.

摘要

在酶联免疫吸附测定（ELISA）中，使用从人骨骼肌中提取的纯化柠檬酸可溶性提取物（AEMA）和从兔肌肉丙酮粉中提取的磷酸盐缓冲盐水提取物（EMA）来包被聚苯乙烯珠。在54例重症肌无力患者中，有14例检测结果呈阳性。6例胸腺瘤患者中有5例抗体水平较高。鉴于在检测中小型胸腺瘤时存在诊断困难，一种用于检测肌肉抗原抗体的灵敏检测方法可能是早期检测肿瘤的重要辅助工具。