一种基于载体的牛结核病候选疫苗的安全性和保护效力
Safety and Protective Efficacy of a Candidate Vector-Based Vaccine for Bovine Tuberculosis.
作者信息
Abay Zhandos, Nurpeisova Ainur, Shorayeva Kamshat, Sadikaliyeva Sandugash, Yespembetov Bolat, Syrym Nazym, Sarmykova Makhpal, Jekebekov Kuanysh, Abitayev Ruslan, Tokkarina Gaukhar, Kalimolda Elina, Absatova Zharkinay, Moldagulova Sabina, Yoo Han Sang, Kassenov Markhabat, Zakarya Kunsulu, Abduraimov Yergali
机构信息
Research Institute for Biological Safety Problems, Guardeyskiy uts 080409, Kazakhstan.
Department of Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
出版信息
Vaccines (Basel). 2023 Jul 4;11(7):1199. doi: 10.3390/vaccines11071199.
This study presents the results of a survey of the safety and protective efficacy of a candidate vector-based vaccine for bovine tuberculosis, using an influenza vector with the NS1 mutation and expressing M. bovis protective antigens ESAT-6 and TB10.4. We vaccinated Balb/c outbred mice two times at 21 days apart. Our experimental design includes mice immunised with the candidate vaccine with or without adjuvant 15% Montanide Gel. The candidate vaccine's safety was determined by biometric analysis, and protective efficacy was assessed by bacteriological and histological experiments following a virulent M. bovis-8 strain challenge. Our data indicated that the adjuvant-free version of the vaccine ensured complete protection from the M. bovis-8 infection in mice.
本研究展示了一项关于基于载体的牛结核病候选疫苗安全性和保护效力的调查结果，该疫苗使用具有NS1突变且表达牛分枝杆菌保护性抗原ESAT-6和TB10.4的流感病毒载体。我们以21天的间隔对远交系Balb/c小鼠进行了两次接种。我们的实验设计包括用含或不含15% Montanide Gel佐剂的候选疫苗免疫小鼠。通过生物统计学分析确定候选疫苗的安全性，并在强毒牛分枝杆菌-8菌株攻击后通过细菌学和组织学实验评估保护效力。我们的数据表明，无佐剂版本的疫苗可确保小鼠完全免受牛分枝杆菌-8感染。
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