Bhatnagar Noopur, Kim Ki-Hye, Subbiah Jeeva, Muhammad-Worsham Sakinah, Park Bo Ryoung, Liu Rong, Grovenstein Phillip, Wang Bao-Zhong, Kang Sang-Moo
Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30302, USA.
Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Vaccines (Basel). 2023 Jul 6;11(7):1209. doi: 10.3390/vaccines11071209.
With concerns about the efficacy of repeat annual influenza vaccination, it is important to better understand the impact of priming vaccine immunity and develop an effective vaccination strategy. Here, we determined the impact of heterologous prime-boost vaccination on inducing broader protective immunity compared to repeat vaccination with the same antigen. The primed mice that were intramuscularly boosted with a heterologous inactivated influenza A virus (H1N1, H3N2, H5N1, H7N9, H9N2) vaccine showed increased strain-specific hemagglutination inhibition titers against prime and boost vaccine strains. Heterologous prime-boost vaccination of mice with inactivated viruses was more effective in inducing high levels of IgG antibodies specific for groups 1 and 2 hemagglutinin stalk domains, as well as cross-protection, compared to homologous vaccination. Both humoral and T cell immunity were found to play a critical role in conferring cross-protection by heterologous prime-boost vaccination. These results support a strategy to enhance cross-protective efficacy by heterologous prime-boost influenza vaccination.
鉴于对每年重复接种流感疫苗效果的担忧，更好地理解启动疫苗免疫的影响并制定有效的疫苗接种策略非常重要。在此，我们确定了与用相同抗原重复接种相比，异源初免-加强接种对诱导更广泛保护性免疫的影响。用异源灭活甲型流感病毒（H1N1、H3N2、H5N1、H7N9、H9N2）疫苗进行肌肉注射加强免疫的初免小鼠，针对初免和加强疫苗毒株的株特异性血凝抑制效价有所提高。与同源接种相比，用灭活病毒对小鼠进行异源初免-加强接种在诱导针对第1组和第2组血凝素茎域的高水平IgG抗体以及交叉保护方面更有效。发现体液免疫和T细胞免疫在通过异源初免-加强接种赋予交叉保护方面都起着关键作用。这些结果支持通过异源初免-加强流感疫苗接种提高交叉保护效力的策略。
