在新型重组辣椒叶脉坏死病毒的 5'UTR 中增加 15 个核苷酸对于感染至关重要。

The Additional 15 nt of 5' UTR in a Novel Recombinant Isolate of Chilli Veinal Mottle Virus in L. Is Crucial for Infection.

机构信息

College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.

出版信息

Viruses. 2023 Jun 23;15(7):1428. doi: 10.3390/v15071428.

DOI:10.3390/v15071428

PMID:37515116

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10384581/

Abstract

An isolate of chilli veinal mottle virus (ChiVMV; genus ) of L. from southwest China (ChiVMV-YunN/Yuxi) was identified and sequenced (GenBank: OP404087). Comparison with other ChiVMV isolates and recombination analyses suggested a recombinant origin. The most significant recombination event among all 21 complete ChiVMV isolates was an ending breakpoint at 1408-1488 for ChiVMV-YunN/Yuxi with ChiVMV-TaiW and ChiVMV-YunN/Ca operating as the respective major and minor parents. Interestingly, the 5' UTR of ChiVMV-YunN/Yuxi is 15 nucleotides ('AAAAATAAAACAACC') longer than other reported isolates. A full-length clone of ChiVMV-YunN/Yuxi was constructed and was shown to be infectious in . The additional 15 nt of 5' UTR in ChiVMV-YunN/Yuxi was stable when transmitted through three generations. Experiments with modified clones showed that the additional 15 nt are essential for infection by this isolate.

摘要

从中国西南部分离到一种辣椒叶脉斑驳病毒（ChiVMV；属）的 L. 菌株，并对其进行了鉴定和测序（GenBank: OP404087）。与其他 ChiVMV 分离株的比较和重组分析表明，该病毒具有重组起源。在所有 21 个完整的 ChiVMV 分离株中，最重要的重组事件是 ChiVMV-YunN/Yuxi 的终止断点位于 1408-1488 位，ChiVMV-TaiW 和 ChiVMV-YunN/Ca 分别作为主要和次要亲本。有趣的是，与其他报道的分离株相比，ChiVMV-YunN/Yuxi 的 5'UTR 长 15 个核苷酸（'AAAAATAAAACAACC'）。构建了 ChiVMV-YunN/Yuxi 的全长克隆，并证明其在 . 中具有感染性。在通过三代传递时，ChiVMV-YunN/Yuxi 中的额外 15nt 5'UTR 是稳定的。使用修饰后的克隆进行的实验表明，对于该分离株的感染，这额外的 15nt 是必不可少的。