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基于 GPE 疫苗株的两种嵌合病毒的构建与效力评价。

Generation and Efficacy of Two Chimeric Viruses Derived from GPE Vaccine Strain as Classical Swine Fever Vaccine Candidates.

机构信息

Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Hokkaido, Japan.

Faculty of Veterinary Medicine, College of Agriculture, Can Tho University, Can Tho 900000, Vietnam.

出版信息

Viruses. 2023 Jul 20;15(7):1587. doi: 10.3390/v15071587.

DOI:10.3390/v15071587
PMID:37515273
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10384557/
Abstract

A previous study proved that vGPE mainly maintains the properties of classical swine fever (CSF) virus, which is comparable to the GPE vaccine seed and is a potentially valuable backbone for developing a CSF marker vaccine. Chimeric viruses were constructed based on an infectious cDNA clone derived from the live attenuated GPE vaccine strain as novel CSF vaccine candidates that potentially meet the concept of differentiating infected from vaccinated animals (DIVA) by substituting the glycoprotein E of the GPE vaccine strain with the corresponding region of non-CSF pestiviruses, either pronghorn antelope pestivirus (PAPeV) or Phocoena pestivirus (PhoPeV). High viral growth and genetic stability after serial passages of the chimeric viruses, namely vGPE/PAPeV E and vGPE/PhoPeV E, were confirmed in vitro. In vivo investigation revealed that two chimeric viruses had comparable immunogenicity and safety profiles to the vGPE vaccine strain. Vaccination at a dose of 10 TCID with either vGPE/PAPeV E or vGPE/PhoPeV E conferred complete protection for pigs against the CSF virus challenge in the early stage of immunization. In conclusion, the characteristics of vGPE/PAPeV E and vGPE/PhoPeV E affirmed their properties, as the vGPE vaccine strain, positioning them as ideal candidates for future development of a CSF marker vaccine.

摘要

先前的研究证明 vGPE 主要保持经典猪瘟病毒（CSF）的特性，这与 GPE 疫苗种子相当，是开发 CSF 标记疫苗的有潜力的宝贵骨架。基于源自活减毒 GPE 疫苗株的感染性 cDNA 克隆构建嵌合病毒，作为新型 CSF 疫苗候选物，通过用非 CSF 瘟病毒（例如叉角羚瘟病毒（PAPeV）或海狮瘟病毒（PhoPeV）的相应区域替换 GPE 疫苗株的糖蛋白 E，潜在地满足区分感染动物和接种动物的概念（DIVA）。嵌合病毒，即 vGPE/PAPeV E 和 vGPE/PhoPeV E，在体外连续传代后证实具有高病毒生长和遗传稳定性。体内研究表明，两种嵌合病毒与 GPE 疫苗株具有相当的免疫原性和安全性。以 10 TCID 的剂量接种 vGPE/PAPeV E 或 vGPE/PhoPeV E，可在免疫早期为猪提供针对 CSF 病毒攻毒的完全保护。总之，vGPE/PAPeV E 和 vGPE/PhoPeV E 的特性证实了它们作为 GPE 疫苗株的性质，使它们成为未来 CSF 标记疫苗开发的理想候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/cc7caf313348/viruses-15-01587-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/b33ce3192f3d/viruses-15-01587-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/cfd07ccaff30/viruses-15-01587-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/218670db12f1/viruses-15-01587-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/23eb3351a612/viruses-15-01587-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/f596ce3b576f/viruses-15-01587-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/cc7caf313348/viruses-15-01587-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/b33ce3192f3d/viruses-15-01587-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/cfd07ccaff30/viruses-15-01587-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/218670db12f1/viruses-15-01587-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/23eb3351a612/viruses-15-01587-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/f596ce3b576f/viruses-15-01587-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7776/10384557/cc7caf313348/viruses-15-01587-g006.jpg

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