Liu Qiao, Zhang Jiarui, Zhang Fuqin, Zhang Wei, Gong Li
Department of Pathology, The Second Affiliated Hospital of Air Force Medical University, Xi'an 710038, China.
Department of Pathology, The Second Affiliated Hospital of Air Force Medical University, Xi'an 710038, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Aug;39(8):686-692.
Objective To identify the possibility of IgG Fc binding protein (FCGBP) acting as a prognostic marker of low-grade glioma (LGG) and its correlation with immune infiltration. Methods The expression of FCGBP was analyzed in pan-cancer using The Cancer Genome Atlas (TCGA), Genotypic tissue expression (GTEX), and China Glioma Genome Atlas (CGGA) database. Then, GSE15824 and GSE68848 datasets were selected for further verification. And gene expression Profile Interaction analysis (GEPIA) database and R language were used to analyze the relationship between FCGBP and survival prognosis. Metascape and GSEA were used for functional annotation and enrichment analysis. Finally, the expression of FCGBP gene in LGG immune microenvironment and its correlation with immune cells were analyzed by TIMER database. Results FCGBP was highly expressed in LGG tissues, indicating poor prognosis of LGG patients. Receiver operating characteristic (ROC) curve analysis and COX analysis showed that FCGBP was an independent risk factor for the prognosis of LGG. Moreover, Gene Ontology (GO) demonstrated that FCGBP was involved in cell metabolism, localization, positive, and negative regulation of biological processes, as well as biological adhesion, response to viral and microbial stimulation, and inflammation. GSEA pathway enrichment analysis showed that FCGBP was significantly correlated with Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway, Toll-like receptor (TLR) pathway, chemokine pathway, and P53 pathway. In addition, FCGBP expression was positively correlated with the expression of most immune cells in the immune microenvironment of LGG. Conclusion The high expression of FCGBP in LGG is a risk factor for survival and prognosis, and it is positively correlated with the expression of immune cells.
目的 确定免疫球蛋白G Fc结合蛋白（FCGBP）作为低级别胶质瘤（LGG）预后标志物的可能性及其与免疫浸润的相关性。方法 使用癌症基因组图谱（TCGA）、基因型组织表达（GTEX）和中国胶质瘤基因组图谱（CGGA）数据库分析泛癌中FCGBP的表达。然后，选择GSE15824和GSE68848数据集进行进一步验证。利用基因表达谱交互分析（GEPIA）数据库和R语言分析FCGBP与生存预后的关系。使用Metascape和基因集富集分析（GSEA）进行功能注释和富集分析。最后，通过TIMER数据库分析FCGBP基因在LGG免疫微环境中的表达及其与免疫细胞的相关性。结果 FCGBP在LGG组织中高表达，提示LGG患者预后不良。受试者工作特征（ROC）曲线分析和COX分析表明，FCGBP是LGG预后的独立危险因素。此外，基因本体论（GO）显示FCGBP参与细胞代谢、定位、生物过程的正调控和负调控，以及生物黏附、对病毒和微生物刺激的反应和炎症。GSEA通路富集分析表明，FCGBP与Janus激酶/信号转导和转录激活因子（JAK/STAT）通路、Toll样受体（TLR）通路、趋化因子通路和P53通路显著相关。此外，FCGBP表达与LGG免疫微环境中大多数免疫细胞的表达呈正相关。结论 FCGBP在LGG中的高表达是生存和预后的危险因素，且与免疫细胞的表达呈正相关。
