Área Académica de Ciencias de la Tierra y Materiales, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo Km. 4.5, 42184, Pachuca, Mexico.
Instituto Nacional de Investigaciones Nucleares; Carr. México-Toluca s/n La Marquesa, C.P. 52750, Ocoyoacac, Estado de México, México.
J Mater Sci Mater Med. 2023 Jul 29;34(8):40. doi: 10.1007/s10856-023-06744-0.
The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH) and (NH)HPO, incorporating MnCl to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations. Samples were characterized by the X-Ray Diffraction technique, which revealed the diffraction peaks that corresponded to the hexagonal and monoclinic phase of the Hap; it was observed that the average size of crystallite decreased from 23.67 to 22.69 nm as the concentration of Mn increased. TEM shows that in all samples, there are two distributions of particle sizes; one corresponds to nanorods with several tens of nanometers in length, and the other in which the diameter and length are very close. FTIR analysis revealed absorption bands corresponding to the PO and OH groups characteristic of the Hap. It was possible to establish a substitution mechanism between the Mn and the ions of Ca of the Hap. From the Alamar blue test, a cell viability of 86.88% ± 5 corresponding to the sample of Hap at 1.5 %wt Mn was obtained, considered non-cytotoxic according to ISO 10993-5. It also evaluated and demonstrated the good osteoinductive properties of the materials, which were verified by histology and immunofluorescence expression of osteogenic markers. Adhesion, viability, biocompatibility and osteoinductive properties, make these materials candidates for future applications in bone tissue engineering with likely uses in regenerative medicine.
本工作研究了 Mn 掺杂对通过水热法在 200°C 下 24 小时合成的 Hap 的晶体结构的影响，所用原料为 Ca(OH)2 和 (NH4)2HPO4，并加入 MnCl2，Mn 的浓度分别为 0.1、0.5、1.0、1.5 和 2.0wt%。样品通过 X 射线衍射技术进行了表征，该技术揭示了对应于 Hap 的六方相和单斜相的衍射峰；观察到随着 Mn 浓度的增加，晶粒的平均尺寸从 23.67 减小到 22.69nm。TEM 显示，在所有样品中，都存在两种粒径分布；一种对应于几十纳米长的纳米棒，另一种则是直径和长度非常接近的纳米棒。FTIR 分析揭示了对应于 Hap 的 PO 和 OH 基团特征的吸收带。可以建立 Mn 与 Hap 中 Ca 离子之间的取代机制。从 Alamar 蓝测试中，可以得到细胞活力为 86.88%±5，对应于 1.5wt%Mn 的 Hap 样品，根据 ISO 10993-5 被认为是非细胞毒性的。它还评估并证明了材料的良好成骨诱导特性，这些特性通过组织学和成骨标志物的免疫荧光表达得到了验证。粘附性、生存能力、生物相容性和成骨诱导特性使这些材料成为未来在骨组织工程中应用的候选材料，可能在再生医学中有潜在用途。
