Biosensor Research Institute, Department of Fine Chemistry, Seoul National University of Science and Technology (Seoul Tech), Gongneung-ro 232, Nowon-gu, Seoul 01811, Republic of Korea.
Department of Biotechnology, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode 637 215, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2021 Jul;126:112170. doi: 10.1016/j.msec.2021.112170. Epub 2021 May 6.
Manganese-doped mesoporous hydroxyapatite (MnHAp) nanorods, a bio-apatite were synthesized via pyridinium chloride mediated microwave approach using bio-waste Donax variabilis seashells to treat orthopedic infections. This is the first report on using pyridinium chloride mediated mesoporous MnHAp nanorods synthesis. Pure and Mn doped HAp samples were examined using Raman spectroscopy, X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) studies to confirm the prepared HAp nanorods. Furthermore, the fabrication of manganese-doped HAp was successful with the formation of a hexagonal crystal lattice without disturbing the HAp phase. It is because, at the time of synthesis, PO ions form an electrostatic interaction with the Mn ions. Furthermore, Mn-doped HAp samples showed a reduction in their sizes of 15, 10-15, 5-10 nm width, and 80-100, 10-15, 20-30 nm length with varied pore diameters and surface area. The pure HAp, MnHAp-1, MnHAp-2, and MnHAp-3 nanorods disclose the surface area of 39.4, 18.0, 49.2, and 80.4 m g, with a pore volume of 0.0102, 0.0047, 0.0143, and 0.0447 cm g, the corresponding pore diameter was estimated to be 6, 7, 6, and 4 nm, respectively. Moreover, antibacterial activity reveals effective bactericidal action against infections causing pathogens whereas cytotoxicity examination (MTT assay), and zebrafish results reveal their non-toxic behavior. Therefore, it is evident from the study, that rapid fabrication of mesoporous and diverse structured MnHAp nanorods could be convenient with pyridinium chloride enabled microwave-assisted method as a bactericidal biomaterial for implant applications.
锰掺杂介孔羟基磷灰石（MnHAp）纳米棒，一种生物磷灰石，通过使用生物废弃物扇贝多肽介导的微波方法合成，以治疗骨科感染。这是首次报道使用吡啶盐酸盐介导的介孔 MnHAp 纳米棒合成。通过拉曼光谱、X 射线粉末衍射（XRD）和傅里叶变换红外光谱（FTIR）研究来检测纯 Mn 掺杂 HAp 样品和纯 Mn 掺杂 HAp 样品，以确认制备的 HAp 纳米棒。此外，MnHAp 的成功合成是由于 PO 离子与 Mn 离子形成静电相互作用，而没有干扰 HAp 相。此外，Mn 掺杂 HAp 样品的尺寸减小到 15、10-15、5-10nm 宽，80-100、10-15、20-30nm 长，具有不同的孔径和表面积。纯 HAp、MnHAp-1、MnHAp-2 和 MnHAp-3 纳米棒的比表面积分别为 39.4、18.0、49.2 和 80.4m2/g，孔体积分别为 0.0102、0.0047、0.0143 和 0.0447cm3/g，相应的孔径分别估计为 6、7、6 和 4nm。此外，抗菌活性揭示了对感染病原体的有效杀菌作用，而细胞毒性（MTT 测定）和斑马鱼结果表明其无毒行为。因此，从研究中可以明显看出，通过使用吡啶盐酸盐促进的微波辅助方法，可以快速制备介孔和多样化结构的 MnHAp 纳米棒，作为用于植入物应用的杀菌生物材料。
