水螅神经元活动和行为的双光子操控。

Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris.

机构信息

Neurotechnology Center, Department Biological Sciences, Columbia University, New York, NY 10027, USA.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102453. doi: 10.1016/j.xpro.2023.102453. Epub 2023 Jul 28.

DOI:10.1016/j.xpro.2023.102453

PMID:37515760

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10400962/

Abstract

The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments.

摘要

钙成像技术的引入使得腔肠动物（如水螅）成为研究神经元活动和行为的有价值的模式生物。在这里，我们提出了一个全面的方案来对水螅的神经元活动和行为进行成像和操作。我们描述了宽场成像和双光子模拟以及神经元消融的步骤。然后，我们详细介绍了成像行为和消融后分析。我们解决了在实验准备和执行过程中可能出现的挑战。