在无菌琼脂平板上用青枯菌接种拟南芥幼苗。

Inoculation of Arabidopsis seedlings with Ralstonia solanacearum in sterile agar plates.

机构信息

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102474. doi: 10.1016/j.xpro.2023.102474. Epub 2023 Jul 28.

DOI:10.1016/j.xpro.2023.102474

PMID:37515761

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10400951/

Abstract

Ralstonia solanacearum invades plants through their roots and causes devastating bacterial diseases in multiple crops. Here, we present a versatile inoculation assay in Arabidopsis thaliana seedlings grown in sterile agar plates. We describe steps for plant preparation, bacterial inoculation, tissue sampling, and bacterial quantification. This protocol can be used for accurate assessment of bacterial colonization and observation of plant response to infection. For complete details on the use and execution of this protocol, please refer to Dindas et al. (2022)..

摘要

青枯雷尔氏菌通过植物根部入侵，并在多种作物中引起破坏性的细菌性疾病。在这里，我们在无菌琼脂平板上生长的拟南芥幼苗中展示了一种多功能的接种检测方法。我们描述了植物准备、细菌接种、组织取样和细菌定量的步骤。该方案可用于准确评估细菌定植和观察植物对感染的反应。有关该方案使用和执行的完整详细信息，请参阅 Dindas 等人。（2022 年）。