College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
Food Chem. 2023 Dec 15;429:136965. doi: 10.1016/j.foodchem.2023.136965. Epub 2023 Jul 25.
It is important to detect the presence of biogenic amines (BAs) as indicators of food freshness. The purpose of this study was to develop a novel ultrasonic-microwave synergistic supramolecular solvent liquid-liquid microextraction based on solidification of floating organic droplet (UMS-SUPRAS-SFO-LLME) combined with high-performance liquid chromatography for the determination of BAs. The physical properties and microstructure of SUPRAS based on 1-dodecanol and tetrahydrofuran were studied, and the extraction conditions such as the SUPRAS volume, the UMS process, and the centrifugal conditions were optimized. The results for the extraction kinetics and thermodynamics showed that UMS-SUPRAS-SFO-LLME is a spontaneous, endothermic diffusion process. The linear ranges of this method are 0.1-2.0 × 10 ng·mL (R > 0.994), the limits of detection are 4.0 × 10-6.0 × 10 ng·mL, and the recoveries were 96.28-103.15%. Compared with existing analysis methods, UMS-SUPRAS-SFO-LLME is a sensitive, green and economical sample pretreatment method for analyzing the enrichment of BAs in beer and fish.
检测生物胺 (BAs) 的存在是判断食品新鲜度的重要指标。本研究旨在开发一种新型超声-微波协同超分子溶剂固态浮油液液微萃取（UMS-SUPRAS-SFO-LLME）结合高效液相色谱法测定 BAs。研究了基于 1-十二醇和四氢呋喃的 SUPRAS 的物理性质和微观结构，并优化了 SUPRAS 体积、UMS 过程和离心条件等萃取条件。萃取动力学和热力学结果表明，UMS-SUPRAS-SFO-LLME 是一种自发的、吸热扩散过程。该方法的线性范围为 0.1-2.0×10 ng·mL（R＞0.994），检出限为 4.0×10-6.0×10 ng·mL，回收率为 96.28-103.15%。与现有分析方法相比，UMS-SUPRAS-SFO-LLME 是一种灵敏、绿色、经济的样品预处理方法，可用于分析啤酒和鱼肉中 BAs 的富集情况。
