The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Guangxi Innovates Medical Technology Co., Ltd. Lipu, Guangxi, 546600, China.
BMC Vet Res. 2023 Jul 29;19(1):98. doi: 10.1186/s12917-023-03662-9.
Neonatal calf diarrhea (NCD) is typically treated with antibiotics, while long-term application of antibiotics induces drug resistance and antibiotic residues, ultimately decreasing feed efficiency. Pueraria polysaccharide (PPL) is a versatile antimicrobial, immunomodulatory, and antioxidative compound. This study aimed to compare the therapeutic efficacy of different doses of PPL (0.2, 0.4, 0.8 g/kg body weight (BW)) and explore the effect of plasma metabolites in diarrheal calves by the best dose of PPL.
PPL could effectively improve the daily weight gain, fecal score, and dehydration score, and the dosage of 0.4 g/kg BW could reach curative efficacy against calf diarrhea (with effective rates 100.00%). Metabolomic analysis suggested that diarrhea mainly affect the levels of taurocholate, DL-lactate, LysoPCs, and intestinal flora-related metabolites, trimethylamine N-oxide; however, PPL improved liver function and intestinal barrier integrity by modulating the levels of DL-lactate, LysoPC (18:0/0:0) and bilirubin, which eventually attenuated neonatal calf diarrhea. It also suggested that the therapeutic effect of PPL is related to those differential metabolites in diarrheal calves.
The results showed that 0.4 g/kg BW PPL could restore the clinical score of diarrhea calves by improving the blood indexes, biochemical indexes, and blood metabolites. And it is a potential medicine for the treatment of calf diarrhea.
新生犊牛腹泻（NCD）通常采用抗生素治疗，而长期应用抗生素会导致耐药性和抗生素残留，最终降低饲料效率。葛根多糖（PPL）是一种用途广泛的抗菌、免疫调节和抗氧化化合物。本研究旨在比较不同剂量的 PPL（0.2、0.4、0.8 g/kg 体重（BW））的治疗效果，并通过 PPL 的最佳剂量探索其对腹泻犊牛血浆代谢物的影响。
PPL 能有效提高日增重、粪便评分和脱水评分，0.4 g/kg BW 的剂量可达到治疗犊牛腹泻的疗效（有效率 100.00%）。代谢组学分析表明，腹泻主要影响牛磺胆酸、DL-乳酸、LysoPCs 和与肠道菌群相关的代谢物三甲胺氮氧化物的水平；然而，PPL 通过调节 DL-乳酸、LysoPC（18:0/0:0）和胆红素的水平来改善肝功能和肠道屏障完整性，从而减轻新生犊牛腹泻。这也表明 PPL 的治疗效果与腹泻犊牛的这些差异代谢物有关。
结果表明，0.4 g/kg BW PPL 通过改善血液指标、生化指标和血液代谢物可以恢复腹泻犊牛的临床评分。它是一种治疗犊牛腹泻的潜在药物。
