Wang Rongjin, Sun Yuzhen, Wang Meiyuan, Li Hanlin, Liu Shu, Liu Zhongying
School of Pharmaceutical Sciences, Jilin University, Changchun, China.
National Center of Mass Spectrometry in Changchun & Jilin Provincial Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Phytother Res. 2023 Oct;37(10):4801-4818. doi: 10.1002/ptr.7947. Epub 2023 Jul 30.
Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves (ESL) are widely used to treat ischemic stroke (IS); however, the specific mechanism remains unclear. The microbiota-gut-brain axis plays a critical role in IS and has become a potential therapeutic target. This study aimed to reveal and verify the therapeutic effect of ESL on IS through the microbiota-gut-brain axis. Ultra-high-performance liquid chromatography coupled with mass spectrometry-based untargeted/targeted metabolomics combined with 16S rRNA microbiota sequencing strategy were used to investigate the regulatory effect of ESL on the metabolism and intestinal microenvironment after IS. Lactobacillus reuteri and Clostridium butyricum were used to treat rats with IS to verify that elevated levels of probiotics are key factors in the therapeutic effect of ESL. The results showed that IS significantly altered the accumulation of 41 biomarkers, while ESL restored their concentrations back to normal. Moreover, ESL alleviated the dysbiosis of gut microbiota brought on by IS, by reducing the abundance of pathogens and increasing the abundance of probiotics (e.g., Lactobacillus reuteri and Clostridium butyricum); this could reduce post-stroke injury, thereby having a certain protective effect on IS. This study reveals that ESL plays an important role in treating IS through the microbiota-gut-brain axis, maintaining metabolic homeostasis in vivo.
刺五加叶（ESL）被广泛用于治疗缺血性中风（IS）；然而，其具体机制仍不清楚。微生物群-肠道-脑轴在缺血性中风中起关键作用，并已成为一个潜在的治疗靶点。本研究旨在通过微生物群-肠道-脑轴揭示并验证刺五加叶对缺血性中风的治疗作用。采用超高效液相色谱联用基于质谱的非靶向/靶向代谢组学结合16S rRNA微生物群测序策略，研究刺五加叶对缺血性中风后代谢和肠道微环境的调节作用。用罗伊氏乳杆菌和丁酸梭菌治疗缺血性中风大鼠，以验证益生菌水平升高是刺五加叶治疗作用的关键因素。结果表明，缺血性中风显著改变了41种生物标志物的积累，而刺五加叶使其浓度恢复正常。此外，刺五加叶减轻了缺血性中风引起的肠道微生物群失调，减少了病原体的丰度，增加了益生菌（如罗伊氏乳杆菌和丁酸梭菌）的丰度；这可以减少中风后的损伤，从而对缺血性中风具有一定的保护作用。本研究表明，刺五加叶通过微生物群-肠道-脑轴在治疗缺血性中风中发挥重要作用，维持体内代谢稳态。
