Gębalski Jakub, Małkowska Milena, Kiełkowska Ewa, Graczyk Filip, Wnorowska Sylwia, Hołyńska-Iwan Iga, Strzemski Maciej, Wójciak Magdalena, Załuski Daniel
Department of Pharmaceutical Botany and Pharmacognosy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
Department of Medical Chemistry, Medical University of Lublin, 20-093 Lublin, Poland.
Int J Mol Sci. 2025 Jun 12;26(12):5619. doi: 10.3390/ijms26125619.
(ES) has been used in traditional medicine for immune-boosting, stress-reducing, and endurance-enhancing properties. In this study, the chemical composition and biological activity of polar and non-polar fractions obtained from 75% methanol roots extract were evaluated. Spectrophotometric methods were used to assess the antioxidant (DPPH, ABTS, FRAP, CUPRAC, O) and anti-enzymatic (hyaluronidase, acetylcholinesterase, butyrylcholinesterase, α-amylase, and tyrosinase) activities. Metabolic profiling was carried out using HPLC-DAD and UHPLC-DAD/ESI-TOF-MS. The ethyl acetate fraction (EtOAc) showed the highest antioxidant activity with IC values of 82.73 ± 0.065 µg/mL (DPPH) and 9.92 ± 0.17 µg/mL (ABTS). The EtOAc fraction also exhibited strong anti-enzymatic effects against hyaluronidase and α-amylase (125.24 ± 12.29 and 97.34 ± 9.18 µg/mL, resp.). In turn, the hexane fraction exhibited the most potent anti-AChE activity with IC equal 245.72 ± 11.82 µg/mL. The HPLC-DAD analysis revealed the presence of caffeic acid derivatives. These results suggest that the ethyl acetate fraction may have therapeutic relevance in inflammation- and metabolic-related diseases. We perceive the potential of this fraction as a rich source of compounds with an anti-inflammatory activity; however, more advanced research in in vivo model is required.
（ES）因其增强免疫力、减轻压力和提高耐力的特性而被用于传统医学。在本研究中，对从75%甲醇根提取物中获得的极性和非极性馏分的化学成分和生物活性进行了评估。采用分光光度法评估抗氧化剂（DPPH、ABTS、FRAP、CUPRAC、O）和抗酶（透明质酸酶、乙酰胆碱酯酶、丁酰胆碱酯酶、α-淀粉酶和酪氨酸酶）活性。使用HPLC-DAD和UHPLC-DAD/ESI-TOF-MS进行代谢谱分析。乙酸乙酯馏分（EtOAc）表现出最高的抗氧化活性，DPPH的IC值为82.73±0.065μg/mL，ABTS的IC值为9.92±0.17μg/mL。EtOAc馏分对透明质酸酶和α-淀粉酶也表现出较强的抗酶作用（分别为125.24±12.29和97.34±9.18μg/mL）。反过来，己烷馏分表现出最有效的抗AChE活性，IC等于245.72±11.82μg/mL。HPLC-DAD分析显示存在咖啡酸衍生物。这些结果表明，乙酸乙酯馏分可能在炎症和代谢相关疾病中具有治疗意义。我们认为该馏分作为具有抗炎活性的化合物的丰富来源具有潜力；然而，需要在体内模型中进行更深入的研究。
