Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tepetitla de Lardizábal, Tlaxcala, Mexico.
Unidad Profesional Interdisciplinaria de Ingeniería Campus Tlaxcala del Instituto Politécnico Nacional, Guillermo Valle, Tlaxcala, Mexico.
Phytochem Anal. 2023 Dec;34(8):1009-1021. doi: 10.1002/pca.3268. Epub 2023 Jul 30.
Dahlia pinnata Cav. is a flower native to Mexico that has many applications; in particular, its petals have been used for ornamental, food, and medicinal purposes, for example to treat skin rashes and skin cracks. It has been reported that the medicinal properties of plants are generally related to the phytochemical constituents they possess. However, there are few studies on black D. pinnata.
The present study was aimed at qualitatively and quantitatively determining the phytochemical profile of petals from black D. pinnata.
Phytochemicals from Dahlia petals were extracted by consecutive maceration (hexane, dichloromethane, and methanol); then, the extracts were analyzed through colorimetric assays and UV-Vis spectroscopy for qualitative identification and quantification of phytochemical compounds, respectively. The methanolic extract was analyzed by flow injection analysis-electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (FIA-ESI-FTICR-MS) in negative and positive mode.
Quantitative phytochemical profiling of the methanolic extract by UV-Vis spectroscopy indicated high contents of phenolic compounds (34.35 ± 3.59 mg EQ/g plant) and sugars (23.91 ± 1.99 mg EQ/g plant), while the qualitative profiling by FIA-ESI-FTICR-MS allowed the tentative identification of several flavonoids and phenolic acids. Kaempferol-3-rutinoside, pelargonidin-3-(6″-malonylglucoside)-5-glucoside, rutin, kaempferol-3-(2″,3″-diacetyl-4″-p-coumaroylrhamnoside), and myricetin-3-(2‴-galloylrhamnoside) were the main compounds detected.
The results expand our knowledge of the phytochemical constituents of petals from black D. pinnata.
大丽花原产于墨西哥，有许多应用；特别是它的花瓣被用于装饰、食品和药用目的，例如治疗皮疹和皮肤裂缝。据报道，植物的药用特性通常与其所含的植物化学成分有关。然而，关于黑色大丽花的研究很少。
本研究旨在定性和定量测定黑色大丽花花瓣的植物化学成分谱。
通过连续浸提（正己烷、二氯甲烷和甲醇）从大丽花花瓣中提取植物化学成分；然后，通过比色分析和紫外可见光谱分别对提取物进行定性鉴定和植物化学成分的定量分析。用流动注射分析-电喷雾电离-傅里叶变换离子回旋共振质谱（FIA-ESI-FTICR-MS）在正、负模式下分析甲醇提取物。
紫外可见光谱对甲醇提取物进行定量植物化学成分分析表明，酚类化合物（34.35 ± 3.59 mg EQ/g 植物）和糖（23.91 ± 1.99 mg EQ/g 植物）含量较高，而 FIA-ESI-FTICR-MS 的定性分析则允许对几种类黄酮和酚酸进行推测性鉴定。鉴定出的主要化合物有山柰酚-3-芸香糖苷、矢车菊素-3-（6″-丙二酰基葡萄糖苷）-5-葡萄糖苷、芦丁、山柰酚-3-（2″，3″-二乙酰基-4″-对香豆酰基鼠李糖苷）和杨梅素-3-（2‴-没食子酰基鼠李糖苷）。
研究结果扩展了我们对黑色大丽花花瓣植物化学成分的认识。
