Songphaeng Thanatta, Lapmanee Sarawut, Bhubhanil Sakkarin, Momdee Kamonchanok, Rojviriya Catleya, Kitsahawong Kemporn, Chailertvanitkul Pattama, Welbat Jariya U, Morkmued Supawich
Faculty of Dentistry, Pediatrics Division, Department of Preventive Dentistry, Khon Kaen University.
Department of Basic Medical Sciences, Faculty of Medicine, Siam University.
J Oral Sci. 2023 Oct 1;65(4):219-225. doi: 10.2334/josnusd.23-0077. Epub 2023 Jul 31.
The purpose of this in vivo study was to determine the effects of stress-induced depression and antidepressants on depressive-like behavior, microstructure, and histomorphology of the temporomandibular joint (TMJ) using rats.
Experimentally induced depression in rats was created before being treated with two antidepressants; escitalopram (selective-serotonin-reuptake inhibitors) and atomoxetine (norepinephrine-reuptake inhibitors). Micro-computed tomography (Micro-CT) was performed to measure the change in bone volume and bone porosity of the condyle. Further histological evaluation of the condylar cartilage was performed.
Micro-CT scanning revealed a decrease in bone volume in the depression group. The bone porosity percentage significantly increased in both the escitalopram and atomoxetine groups compared with the control group and the depression group. Histopathological analysis showed increased thickness of cartilage layers in the depression group. In the atomoxetine group, there was a significant increase in the pre-hypertrophic and hypertrophic layer thickness and cell count, but a significant decrease in proteoglycans.
The present study findings indicated the change in TMJ characteristics, especially on the superficial part of the condylar head in the depression group. Concerning the applicability of the different antidepressants, depression with the treatment of atomoxetine has the most disadvantages due to bone porosity and cartilaginous condyle changes.
本体内研究旨在利用大鼠确定应激诱导的抑郁及抗抑郁药对颞下颌关节（TMJ）的抑郁样行为、微观结构和组织形态学的影响。
在对大鼠进行两种抗抑郁药治疗之前，先通过实验诱导其产生抑郁；这两种抗抑郁药分别是艾司西酞普兰（选择性5-羟色胺再摄取抑制剂）和托莫西汀（去甲肾上腺素再摄取抑制剂）。采用微型计算机断层扫描（Micro-CT）测量髁突的骨体积和骨孔隙率的变化。对髁突软骨进行进一步的组织学评估。
Micro-CT扫描显示抑郁组骨体积减少。与对照组和抑郁组相比，艾司西酞普兰组和托莫西汀组的骨孔隙率百分比均显著增加。组织病理学分析显示抑郁组软骨层厚度增加。在托莫西汀组，前肥大层和肥大层厚度及细胞计数显著增加，但蛋白聚糖显著减少。
本研究结果表明颞下颌关节特征发生了变化，尤其是抑郁组髁突头部的表层部分。关于不同抗抑郁药的适用性，由于骨孔隙率和髁突软骨变化，托莫西汀治疗的抑郁具有最多的不利之处。
