Department of Pathology, University of Medicine and Pharmacy of Craiova, Romania;
Rom J Morphol Embryol. 2023 Apr-Jun;64(2):165-171. doi: 10.47162/RJME.64.2.06.
Claudins are a family of essential tight junction proteins, abnormally expressed in human carcinomas. The studies that indicated the involvement of claudins in tumor biology and progression suggest the possibility of their utility as markers for diagnosis or prognosis, but also as possible targets for therapy. We investigated 50 prostate adenocarcinomas (PAs) for which we followed the expression of Claudins -3, -4 and -7 in relation to International Society of Urological Pathology (ISUP) grades. We observed the positivity for Claudin-3, Claudin-4, and Claudin-7 in 76%, 74% and 46% of cases. Analysis of the immunoexpression pattern revealed the cytoplasmic and nuclear translocation for Claudins -3 and -4, and only cytoplasmic for Claudin-7. For all claudins investigated, we noted a final staining score with significantly higher values or at the limit of statistical significance for PA belonging to ISUP groups 1-4. The internalization of Claudins -3, -4 and -7 expression, regardless of the degree of PA, indicates their involvement in prostate carcinogenesis. In addition, the similar immunoexpression patterns of the three investigated claudins and their positive linear correlation suggest a coordinated regulation and indicate the possibility of a targeted treatment strategy.
紧密连接蛋白家族成员 Claudin 在人类癌组织中异常表达。Claudin 在肿瘤生物学和进展中的作用的研究提示 Claudin 可作为诊断或预后标志物，并可能成为治疗靶点。我们研究了 50 例前列腺腺癌（PA），分析 Claudin-3、Claudin-4 和 Claudin-7 的表达与国际泌尿病理学会（ISUP）分级的关系。我们观察到 Claudin-3、Claudin-4 和 Claudin-7 在 76%、74%和 46%的病例中呈阳性。免疫组化结果显示 Claudin-3 和 Claudin-4 发生了细胞质和核转位，而 Claudin-7 仅发生了细胞质转位。对于所有研究的 Claudin，我们发现 ISUP 1-4 级 PA 的终末染色评分明显较高或具有统计学意义。Claudin-3、Claudin-4 和 Claudin-7 表达的内化，无论 PA 程度如何，都表明其参与了前列腺癌的发生。此外，三种 Claudin 的免疫组化表达模式相似，且呈正线性相关，提示 Claudin 的表达受到协调调控，并提示靶向治疗策略的可能性。
