Väre P, Loikkanen I, Hirvikoski P, Vaarala M H, Soini Y
Department of Pathology, University of Oulu and Oulu University Hospital, 90014 Oulu, Finland.
Oncol Rep. 2008 Jan;19(1):25-31.
The expression of claudins 1, 2, 3, 4, 5 and 7 was studied in prostate adenocarcinoma and compared to that of non-neoplastic epithelium and Gleason score of the tumors. Additionally, RT-PCR was performed for claudins 2 and 5 in three cases. Strong immunoreactivity of claudins 1, 3, 4 and 7 was seen in prostate adenocarcinoma while expression of claudins 2 and 5 was weaker. In relation to non-neoplastic glands, expression of claudins 2 and 5 was diminished. There was a significant association between the Gleason score and claudin 1 and 5 expression, these claudins were more strongly expressed in tumours with a lower Gleason score. A combined lowered claudin expression was associated with a high Gleason score and a poor prognosis. According to the results, there is a strong claudin expression in prostate adenocarcinoma, especially for claudins 3 and 4. In contrast, claudin 2 was low in neoplastic cells compared to non-neoplastic epithelium, suggesting downregulation of synthesis or altered metabolism/assembly of this protein during carcinoma development. The association of lowered claudin 1 and 5 expression and lowered overall claudin expression with tumours of a higher Gleason score suggest that claudins may modulate the histologic features of these tumors and in this way influence their biological behaviour.
研究了紧密连接蛋白1、2、3、4、5和7在前列腺腺癌中的表达，并与非肿瘤上皮及肿瘤的Gleason评分进行比较。此外，对3例病例的紧密连接蛋白2和5进行了逆转录聚合酶链反应（RT-PCR）。在前列腺腺癌中可见紧密连接蛋白1、3、4和7的强免疫反应性，而紧密连接蛋白2和5的表达较弱。与非肿瘤腺体相比，紧密连接蛋白2和5的表达减少。Gleason评分与紧密连接蛋白1和5的表达之间存在显著关联，这些紧密连接蛋白在Gleason评分较低的肿瘤中表达更强。紧密连接蛋白表达联合降低与高Gleason评分及不良预后相关。根据结果，前列腺腺癌中存在紧密连接蛋白的强表达，尤其是紧密连接蛋白3和4。相反，与非肿瘤上皮相比，肿瘤细胞中紧密连接蛋白2含量较低，提示在肿瘤发生过程中该蛋白的合成下调或代谢/组装改变。紧密连接蛋白1和5表达降低以及总体紧密连接蛋白表达降低与高Gleason评分肿瘤的关联表明，紧密连接蛋白可能调节这些肿瘤的组织学特征，进而影响其生物学行为。
