Department of Histology and Embryology, Faculty of Medicine, Near East University, Nicosia, Cyprus;
Rom J Morphol Embryol. 2023 Apr-Jun;64(2):207-213. doi: 10.47162/RJME.64.2.11.
Liver ischemia/reperfusion (IR) often affects distant organs, such as small intestine, kidney, and lung. Coriandrum sativum (CS) has an antioxidant and anti-inflammatory effect on liver damage. The aim of this study was to investigate the anti-inflammatory and antiapoptotic effects of CS extract on small intestine, lung, and kidney after the liver IR injury. Small intestine, lung, and kidney tissues were evaluated and scored in terms of cell degeneration, inflammation, and congestion, as well as caspase-3 (Cas-3) and cluster of differentiation 31 (CD31) immunostainings were carried out. Renal enzymes, creatinine and urea levels were measured biochemically in serum. After IR, a decrease in villi size, diffuse degeneration, epithelial cell shedding and extensive congestion in the capillaries were observed. Meanwhile, the number of degenerated villi and congestion decreased in the IR+CS group. Due to IR, increased congestion was detected in the interalveolar septum of the lungs and in the capillaries between the kidney tubules. It was also observed that the positively stained cells with Cas-3 and CD31 were increased in the lung, kidney, and small intestine tissues of the IR group, and decreased in the IR+CS group. Kidney enzymes, urea and creatinine levels were significantly increased in the IR group and decreased in the IR+CS group. In conclusion, it was observed that liver IR caused changes in distant organs, especially in the small intestine, lung, and kidneys. Damaging effects of IR as well as apoptosis and inflammation were found to be decreased in the groups treated with CS.
肝脏缺血/再灌注（IR）常影响到远处的器官，如小肠、肾脏和肺部。芫荽（Coriandrum sativum，CS）对肝损伤具有抗氧化和抗炎作用。本研究旨在探讨 CS 提取物对肝脏 IR 损伤后小肠、肺和肾脏的抗炎和抗凋亡作用。从细胞变性、炎症和充血的角度评估和评分小肠、肺和肾脏组织，并进行 caspase-3（Cas-3）和分化簇 31（CD31）免疫染色。通过生化方法测量血清中的肾酶、肌酐和尿素水平。IR 后，观察到绒毛大小减小、弥漫性变性、上皮细胞脱落和毛细血管广泛充血。与此同时，IR+CS 组中退化绒毛和充血的数量减少。由于 IR，在肺泡间隔和肾小管之间的毛细血管中观察到充血增加。还观察到，IR 组的肺、肾和小肠组织中 Cas-3 和 CD31 阳性染色细胞增加，而 IR+CS 组则减少。IR 组的肾酶、尿素和肌酐水平显著升高，而 IR+CS 组则降低。总之，观察到肝脏 IR 导致远处器官发生变化，特别是小肠、肺和肾脏。在 CS 治疗组中，观察到 IR 的损伤作用以及凋亡和炎症减少。
