可控应激增加大鼠的快速眼动睡眠：杏仁中央核的调节。

Controllable Stress Increases Rapid Eye Movement Sleep in Rats: Regulation by the Central Nucleus of the Amygdala.

机构信息

Sleep Research Laboratory, Center for Integrative Neuroscience and Inflammatory Diseases, Pathology and Anatomy, Eastern Virginia Medical School, Norfolk, VA 23507, USA.

Sleep Medicine Center, Mental Health Center, Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, 610041 Chengdu, Sichuan, China.

出版信息

J Integr Neurosci. 2023 Jul 20;22(4):100. doi: 10.31083/j.jin2204100.

DOI:10.31083/j.jin2204100

PMID:37519182

Abstract

BACKGROUND

Training with inescapable shock (IS; uncontrollable stressor) is followed by significant decreases in rapid eye movement sleep (REM). However, controllability is important in the effects of stress. We examined the effects of escapable shock (ES; controllable stressor) on sleep and whether the central nucleus of the amygdala (CNA) plays a role in regulating these effects.

METHODS

Six Wistar rats implanted with a cannula located in CNA underwent two days of ES training (20 shock presentations; 0.5 mA; 5.0 s maximum duration; 1.0 min interstimulus interval). Five days later, they were re-exposed to the shock context.

RESULTS

Following shock training, REM was significantly increased in both light and dark periods. Non-REM (NREM) and total sleep (TS) duration were decreased during the light period. Similar effects on REM and NREM were observed following re-exposure to the training context alone. Microinjections of saline into CNA immediately following ES also produced similar increases in REM, whereas microinjections of muscimol (MUS; GABAA (γ-aminobutyric acid) antagonist) subsequent to ES blocked the increases in REM.

CONCLUSIONS

These data, along with previous work with ES and IS, demonstrate that stressor controllability is important in determining how stress impacts sleep. Moreover, the results of the microinjection study indicate that the effects of ES on REM are regulated through the CNA.

摘要

背景

不可逃避的休克（IS；不可控压力源）训练后，快速眼动睡眠（REM）会显著减少。然而，在压力的影响中，可控性是很重要的。我们研究了可逃避的休克（ES；可控压力源）对睡眠的影响，以及杏仁中央核（CNA）在调节这些影响中是否起作用。

方法

六只植入 CNA 导管的 Wistar 大鼠进行了两天的 ES 训练（20 次电击呈现；0.5 mA；最大持续时间 5.0 s；刺激间隔 1.0 分钟）。五天后，它们再次暴露在电击环境中。

结果

在电击训练后，REM 在明亮和黑暗期都显著增加。非快速眼动（NREM）和总睡眠时间（TS）在明亮期减少。单独重新暴露于训练环境也会对 REM 和 NREM 产生类似的影响。ES 后立即向 CNA 内注射盐水也会导致 REM 相似的增加，而 ES 后注射 Muscimol（MUS；GABAA（γ-氨基丁酸）拮抗剂）则会阻断 REM 的增加。