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Goody two plasmids: An optimized transient transfection system for AAV vector production.

作者信息

Moreno Velasquez Sergio David, Gerstmann Emma, Grimm Dirk

机构信息

Department of Infectious Diseases/Virology, Section Viral Vector Technologies, Medical Faculty, University of Heidelberg, BioQuant, Center for Integrative Infectious Diseases (CIID), 69120 Heidelberg, Germany.

German Center for Infection Research (DZIF) and German Center for Cardiovascular Research (DZHK), Partner Site Heidelberg, 69120 Heidelberg, Germany.

出版信息

Mol Ther Methods Clin Dev. 2023 Jul 13;30:191-193. doi: 10.1016/j.omtm.2023.06.010. eCollection 2023 Sep 14.

DOI:10.1016/j.omtm.2023.06.010
PMID:37519408
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10371777/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f466/10371777/15477628d6d5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f466/10371777/15477628d6d5/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f466/10371777/15477628d6d5/gr1.jpg

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Fantastic AAV Gene Therapy Vectors and How to Find Them-Random Diversification, Rational Design and Machine Learning.神奇的腺相关病毒基因治疗载体及其寻找方法——随机多样化、合理设计与机器学习
Pathogens. 2022 Jul 3;11(7):756. doi: 10.3390/pathogens11070756.
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Mechanistic model for production of recombinant adeno-associated virus via triple transfection of HEK293 cells.
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Mol Ther Methods Clin Dev. 2021 Apr 16;21:642-655. doi: 10.1016/j.omtm.2021.04.006. eCollection 2021 Jun 11.
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Manufacturing Challenges and Rational Formulation Development for AAV Viral Vectors.AAV 病毒载体的制造挑战和合理配方开发。
J Pharm Sci. 2021 Jul;110(7):2609-2624. doi: 10.1016/j.xphs.2021.03.024. Epub 2021 Apr 2.
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Two-Plasmid Packaging System for Recombinant Adeno-Associated Virus.重组腺相关病毒的双质粒包装系统
Biores Open Access. 2020 Oct 16;9(1):219-228. doi: 10.1089/biores.2020.0031. eCollection 2020.
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Nat Rev Genet. 2020 Apr;21(4):255-272. doi: 10.1038/s41576-019-0205-4. Epub 2020 Feb 10.
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