酪胺、酪氨酸及含酪氨酸肽对DNA中胸腺嘧啶二聚体的光敏形成作用。
Photosensitized formation of thymine dimers in DNA by tyramine, tyrosine and tyrosine-containing peptides.
作者信息
Kaneko M, Matsuyama A, Nagata C
出版信息
Nucleic Acids Res. 1979 Mar;6(3):1177-87. doi: 10.1093/nar/6.3.1177.
The formation of Thy-Thy in DNA in the presence of tyramine, tyrosine and tyrosine-containing peptides such as Lys-Tyr and Lys-Tyr-Lys was studied with monochromatic UV irradiation. The formation of Thy-Thy by UV irradiation was enhanced in the presence of these compounds. The action spectrum of the photosensitization has a peak near 280 nm corresponding to the absorption spectrum of tyrosine. The triplet quencher reduced the sensitization substantially. The sensitization in native DNA was more than six times larger than that in denatured DNA. increasing the concentration of salts suppressed the sensitization. The nature of the interaction between DNA and the sensitizer is discussed.
在存在酪胺、酪氨酸以及含酪氨酸的肽（如赖氨酸 - 酪氨酸和赖氨酸 - 酪氨酸 - 赖氨酸）的情况下，通过单色紫外线照射研究了DNA中胸腺嘧啶 - 胸腺嘧啶（Thy - Thy）的形成。在这些化合物存在下，紫外线照射导致的Thy - Thy形成得到增强。光敏化作用光谱在280nm附近有一个峰值，与酪氨酸的吸收光谱相对应。三重态猝灭剂显著降低了光敏化作用。天然DNA中的光敏化作用比变性DNA中的大六倍以上。增加盐的浓度会抑制光敏化作用。文中讨论了DNA与敏化剂之间相互作用的性质。
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