DNA在313nm光敏紫外线照射下构象变化的圆二色性研究。
CD studies of conformational changes of DNA upon photosensitized UV-irradiation at 313 nm.
作者信息
Lang H
出版信息
Nucleic Acids Res. 1975 Feb;2(2):179-83. doi: 10.1093/nar/2.2.179.
Abstract
The role of thymine dimerization for previously reported U.V. induced conformational changes has been proved using acetophenone as sensitizer for the specific thymine dimerization upon irradiation at 313 nm. CD results demonstrate that formation of pyrimidine dimers cause typical conformational changes of the DNA B helix as observed on irradiation at 254 nm. Thus the primary role of adenine photoproducts may be excluded.
摘要
利用苯乙酮作为敏化剂，在313nm波长照射下实现特定胸腺嘧啶二聚化，从而证明了胸腺嘧啶二聚化在先前报道的紫外线诱导构象变化中的作用。圆二色性（CD）结果表明，嘧啶二聚体的形成会导致DNA B螺旋发生典型的构象变化，这与在254nm波长照射下所观察到的情况一致。因此，可以排除腺嘌呤光产物的主要作用。
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本文引用的文献
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Specific formation of thymine dimers in DNA.
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