Keren D F, Di Sante A C, Bordine S L
Am J Clin Pathol. 1986 Mar;85(3):348-52. doi: 10.1093/ajcp/85.3.348.
The recent introduction of high-resolution electrophoresis into many clinical laboratories has expanded the information available about protein abnormalities. While most laboratories interpret high-resolution electrophoresis patterns by direct visual examination of the stained electrophoresis strips, much useful information can be attained by supplemental densitometric scanning of these preparations. In the present report, we detail a method to perform densitometric scanning of high-resolution electrophoresis strips, and compare these results to those performed by standard cellulose acetate electrophoresis. Further, we evaluate the quantitative information by comparing the results from the densitometer scans with nephelometric quantification of specific monoclonal proteins. The present method gives a correlation coefficient of 0.97 when the gamma region densitometric scan is compared to the quantification of IgG by nephelometry in patients with known gamma-migrating IgG monoclonal gammopathies. Further, twofold dilution studies of these specimens showed excellent linearity. By providing objective, reliable, quantitative information, densitometric scanning of high-resolution electrophoresis strips is a useful adjunct to direct visual examination of these specimens.
近期，高分辨率电泳技术在许多临床实验室的应用，拓展了有关蛋白质异常情况的可用信息。虽然大多数实验室通过直接目视检查染色后的电泳条带来解读高分辨率电泳图谱，但对这些标本进行补充光密度扫描可获得许多有用信息。在本报告中，我们详细介绍了一种对高分辨率电泳条带进行光密度扫描的方法，并将这些结果与标准醋酸纤维素电泳的结果进行比较。此外，我们通过将光密度计扫描结果与特定单克隆蛋白的比浊法定量结果进行比较，来评估定量信息。当将已知γ迁移IgG单克隆丙种球蛋白病患者的γ区光密度扫描结果与IgG比浊法定量结果进行比较时，本方法的相关系数为0.97。此外，对这些标本进行的两倍稀释研究显示出良好的线性关系。通过提供客观、可靠的定量信息，高分辨率电泳条带的光密度扫描是对这些标本进行直接目视检查的有用辅助手段。
