Lu Zeyu, Wu Jing, Wu Jing, Zhang Tiejia, Liu Junyang, Mu Qing, Wu Zixian, Zhang Yanjun, Su Rui, Liu Zhihong, Wang Zhiying, Wang Ruijun, Qi Lv, Zhao Yanhong
State Key Laboratory of Animal Genetics and Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Front Vet Sci. 2023 Jul 10;10:1203302. doi: 10.3389/fvets.2023.1203302. eCollection 2023.
Cashmere is the fine bottom hair produced by the secondary hair follicles of the skin. This hair is economically important. Previous studies by our research group have shown that exogenous melatonin (MT) can regulate the periodic growth of secondary hair follicles, induce the secondary development of villi, and alter the expression of some genes related to hair follicle development. Few studies on the regulation of villus growth by MT binding receptors have been published. In this study, MT was implanted subcutaneously behind the ear of Inner Mongolia cashmere goats. RT-qPCR, hybridization, Western blot analysis, immunofluorescence and RNAi techniques were used to investigate the receptors and functions of MT in regulating the development of secondary hair follicles in Inner Mongolia cashmere goats. The results showed that MT binds to the nuclear receptor RORα on dermal papilla stimulates hair follicle development and promotes villus growth. The RORα mRNA expression in the skin of Inner Mongolia cashmere goats was periodic and showed a trend of first increasing and then decreasing. The expression began to increase in February, peaked in April, and reached the lowest level in May. RORα significantly affected the mRNA expression of β- gene, a key gene in hair follicle development, in the presence of MT. It will lay a solid molecular foundation for further research on the regulation mechanism between MT receptor and villus growth and development and to achieve artificial regulation of villus growth time and yield to improve the effect of villus production.
羊绒是皮肤次级毛囊产生的细软绒毛。这种毛发具有重要的经济价值。我们研究小组之前的研究表明，外源性褪黑素（MT）可以调节次级毛囊的周期性生长，诱导绒毛的二次发育，并改变一些与毛囊发育相关基因的表达。关于MT结合受体对绒毛生长调控的研究报道较少。本研究将MT皮下植入内蒙古绒山羊耳后。采用RT-qPCR、杂交、蛋白质免疫印迹分析、免疫荧光和RNA干扰技术，研究MT在调控内蒙古绒山羊次级毛囊发育中的受体及功能。结果表明，MT与毛乳头细胞上的核受体RORα结合，刺激毛囊发育，促进绒毛生长。内蒙古绒山羊皮肤中RORα mRNA表达具有周期性，呈先升高后降低的趋势，2月开始升高，4月达到峰值，5月降至最低水平。在MT存在的情况下，RORα显著影响毛囊发育关键基因β - 基因的mRNA表达。这将为进一步研究MT受体与绒毛生长发育调控机制，实现人工调控绒毛生长时间和产量，提高绒毛生产效益奠定坚实的分子基础。
