Othman Farah Amna, Mat Zin Anani Aila, Zakaria Yusmazura, Nik Salleh Nik Nur Hakimah, Tan Suat Cheng
School of Health Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, 16150, Kelantan, Malaysia.
Pathology Department, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, 16150, Kelantan, Malaysia.
Data Brief. 2023 Jul 15;49:109411. doi: 10.1016/j.dib.2023.109411. eCollection 2023 Aug.
is a medicinal herb that garnered enormous attention in drug discovery for human diseases such as neurodegenerative, cardiovascular, arthritis and hepatitis diseases. Pharmacokinetic study confirmed that the pharmacological actions of this herb are associated with its prominent flavonoid bioactive components. Here, the data set of liquid chromatography-mass spectroscopy (LC-MS), neurological functions, relative organ weight (ROW), hematological, biochemical and histopathological parameters of flavonoid-enriched fraction (FEF)-treated Sprague Dawley (SD) rats were presented. The data set was generated from three study groups namely: Sighting Study, Acute Toxicity Study and Subacute Neurotoxicity Study with study duration of 14 days (for Sighting Study and Acute Toxicity Study) and 28 days (for Subacute Neurotoxicity Study) by strictly following the procedures set in Organisation for Economic Co-operation and Development (OECD) Guidelines 420 and 424 in vivo. Rats in sighting study were treated with dosage of 5, 50, 300 and 2000 mg/kg FEF ( = 1/dosage/gender), respectively, and were observed for mortality, toxicity signs and behavioural changes. The highest dosage at which none of the animal showed sign of mortality in the sighting study was selected as the test dosage for subsequent acute toxicity study ( = 5/dosage/gender). Meanwhile, for subacute neurotoxicity study, SD rats ( = 5/dosage/gender) were treated with repeated dosage of 50 mg/kg for 28 days. Neurological behaviours of treated rats were observed daily, while their body weight were measured weekly. Whole blood was collected at the end of the study via cardiac puncture into ethylenediaminetetraacetic acid (EDTA) tubes for hematological evaluation that included the measurements of red blood cells (RBC), hemoglobin (Hb), packed cell volumes (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet, white blood cells (WBC) count and WBC differentials. Meanwhile, blood serum were collected into slow sand filter (SST) tubes for biochemical evaluation that included measurements of total protein (TP), albumin, bilirubin, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Vital organs such as brain, liver, kidneys, heart, lungs and reproductive organs also were collected, sliced and stained with hematoxylin and eosin (H&E) at the end of the study for histopathological assessments.
是一种草药，在针对神经退行性疾病、心血管疾病、关节炎和肝炎等人类疾病的药物研发中备受关注。药代动力学研究证实，这种草药的药理作用与其显著的黄酮类生物活性成分有关。在此，呈现了用富含黄酮类成分（FEF）处理的斯普拉格-道利（SD）大鼠的液相色谱-质谱联用（LC-MS）数据集、神经功能、相对器官重量（ROW）、血液学、生化和组织病理学参数。该数据集来自三个研究组，即：预试验研究、急性毒性研究和亚急性神经毒性研究，预试验研究和急性毒性研究的持续时间为14天，亚急性神经毒性研究的持续时间为28天，在体内严格遵循经济合作与发展组织（OECD）指南420和424中规定的程序。预试验研究中的大鼠分别用5、50、300和2000 mg/kg FEF（n = 1/剂量/性别）的剂量进行处理，并观察死亡率、毒性体征和行为变化。在预试验研究中未出现动物死亡迹象的最高剂量被选为后续急性毒性研究的试验剂量（n = 5/剂量/性别）。同时，对于亚急性神经毒性研究，SD大鼠（n = 5/剂量/性别）用50 mg/kg的重复剂量处理28天。每天观察处理后大鼠的神经行为，每周测量其体重。在研究结束时，通过心脏穿刺将全血收集到乙二胺四乙酸（EDTA）管中进行血液学评估，包括测量红细胞（RBC）、血红蛋白（Hb）、血细胞比容（PCV）、平均红细胞体积（MCV）、平均红细胞血红蛋白（MCH）、平均红细胞血红蛋白浓度（MCHC）、血小板、白细胞（WBC）计数和白细胞分类。同时，将血清收集到促凝管（SST）中进行生化评估，包括测量总蛋白（TP）、白蛋白、胆红素、碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。在研究结束时，还收集了脑、肝、肾、心、肺和生殖器官等重要器官，切片并用苏木精和伊红（H&E）染色进行组织病理学评估。
