核移植可提高源自细胞质缺陷卵母细胞的胚胎的发育潜力。
Nuclear transfer improves the developmental potential of embryos derived from cytoplasmic deficient oocytes.
作者信息
Liao Zhaodi, Li Yuzhuo, Li Chunyang, Bian Xinyan, Sun Qiang
机构信息
Institute of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, China.
Shanghai Center for Brain Science and Brain-Inspired Technology, Shanghai 201210, China.
出版信息
iScience. 2023 Jul 10;26(8):107299. doi: 10.1016/j.isci.2023.107299. eCollection 2023 Aug 18.
Embryo development after fertilization is largely determined by the oocyte quality, which is in turn dependent on the competence of both the cytoplasm and nucleus. Here, to improve the efficiency of embryo development from developmentally incompetent oocytes, we performed spindle-chromosome complex transfer (ST) between matured (IVM) and matured (IVO) oocytes of the non-human primate rhesus monkey. We observed that the blastocyst rate of embryos derived from transferring the spindle-chromosome complex (SCC) of IVM oocytes into enucleated IVO oocytes was comparable with that of embryos derived from IVO oocytes. After transferring the reconstructed embryos into the uterus of surrogate mothers, two live rhesus monkeys were obtained, indicating that the nuclei of IVM oocytes support both the pre-and post-implantation embryo development of non-human primates.
受精后的胚胎发育很大程度上由卵母细胞质量决定，而卵母细胞质量又取决于细胞质和细胞核的能力。在此，为提高发育能力欠佳的卵母细胞的胚胎发育效率，我们在非人灵长类恒河猴的成熟（体外成熟，IVM）卵母细胞和成熟（体内成熟，IVO）卵母细胞之间进行了纺锤体-染色体复合体转移（ST）。我们观察到，将IVM卵母细胞的纺锤体-染色体复合体（SCC）转移到去核的IVO卵母细胞中所获得的胚胎的囊胚率与来自IVO卵母细胞的胚胎相当。将重构胚胎移植到代孕母亲子宫后，获得了两只存活的恒河猴，这表明IVM卵母细胞的细胞核支持非人灵长类动物植入前和植入后的胚胎发育。
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