Chaudhari Hemangini A, Mahatma Mahesh Kumar, Antala Virali, Radadiya Nidhi, Ukani Piyush, Tomar Rukam Singh, Thawait Lokesh Kumar, Singh Sushmita, Gangadhara K, Sakure Amar, Parihar Akrash
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat 362001 India.
ICAR-Directorate of Groundnut Research, Junagadh, Gujarat 362001 India.
Physiol Mol Biol Plants. 2023 Jun;29(6):829-842. doi: 10.1007/s12298-023-01332-6. Epub 2023 Jul 19.
It is important to have a short period of fresh seed dormancy in some of the groundnut species to counter pre-harvest sprouting (PHS). One of the main causes of PHS is the activation of ethylene-mediated pathways. To determine the effect of ethylene, the study was conducted and alterations in amylase, proteins and fatty acids were observed at the 0, 6, 12, and 24 h stages after ethrel administration. The result showed an increase in amylase activity, and the fatty acids profile showed a unique alteration pattern at different germination stages. Two-dimensional gel electrophoresis (2DGE) revealed differential expression of proteins at each stage. The trypsin digestion following spectral development through UPLC-MS/MS enabled identification of number of differentially expressed proteins. A total of 49 proteins were identified from 2DGE excised spots. The majority were belonged to seed storage-related proteins like Arah1, Arah2, AAI- domain containing protein, conglutin, Arah3/4, arachin, glycinin. Expression of , and genes were further confirmed by qRT-PCR which showed its involvement at transcript level. Up-regulation of is correlated with decreased content of fatty acids during germination. Phytohormone detection revealed decrease in ABA, SA and JA content which are generally inhibitor of seed germination while GA, IAA and kinetin concentration increased revealing positive regulation of seed germination. We present an integrated view of proteomics, phytohormone profile, carbohydrate and lipid metabolism to unravel mechanism of fresh seed dormancy.
The online version contains supplementary material available at 10.1007/s12298-023-01332-6.
对于某些花生品种而言，短暂的新鲜种子休眠期对于防止收获前发芽（PHS）很重要。PHS的主要原因之一是乙烯介导途径的激活。为了确定乙烯的作用，开展了本研究，并在施用乙烯利后的0、6、12和24小时阶段观察淀粉酶、蛋白质和脂肪酸的变化。结果显示淀粉酶活性增加，并且脂肪酸谱在不同发芽阶段呈现出独特的变化模式。二维凝胶电泳（2DGE）揭示了每个阶段蛋白质的差异表达。通过超高效液相色谱-串联质谱（UPLC-MS/MS）进行光谱分析后的胰蛋白酶消化能够鉴定出许多差异表达的蛋白质。从2DGE切下的斑点中共鉴定出49种蛋白质。大多数属于种子储存相关蛋白，如Arah1、Arah2、含AAI结构域的蛋白、凝集素、Arah3/4、花生球蛋白、大豆球蛋白。通过qRT-PCR进一步证实了 、 和 基因的表达，表明它们在转录水平上的参与。 的上调与发芽过程中脂肪酸含量的降低相关。植物激素检测显示脱落酸（ABA）、水杨酸（SA）和茉莉酸（JA）含量降低，它们通常是种子发芽的抑制剂，而赤霉素（GA）、吲哚乙酸（IAA）和激动素浓度增加，表明对种子发芽有正向调节作用。我们提供了蛋白质组学、植物激素谱、碳水化合物和脂质代谢的综合观点，以揭示新鲜种子休眠的机制。
在线版本包含可在10.1007/s12298-023-01332-6获取的补充材料。
