Bellieny-Rabelo Daniel, De Oliveira Eduardo Alves Gamosa, Ribeiro Elaneda Silva, Costa Evenilton Pessoa, Oliveira Antônia Elenir Amâncio, Venancio Thiago Motta
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Brazil.
Núcleo em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé (NUPEM), Campus UFRJ Macaé, Macaé, Brazil.
Sci Rep. 2016 Nov 8;6:36009. doi: 10.1038/srep36009.
Soybean (Glycine max) is a major legume crop worldwide, providing a critical source of protein and oil. The release of the soybean genome fuelled several transcriptome projects comprising multiple developmental stages and environmental conditions. Nevertheless, the global transcriptional patterns of embryonic axes during germination remain unknown. Here we report the analysis of ~1.58 billion RNA-Seq reads from soybean embryonic axes at five germination stages. Our results support the early activation of processes that are critical for germination, such as glycolysis, Krebs cycle and cell wall remodelling. Strikingly, only 3 hours after imbibition there is a preferential up-regulation of protein kinases and transcription factors, particularly from the LOB domain family, implying that transcriptional and post-transcriptional regulation play major roles early after imbibition. Lipid mobilization and glyoxylate pathways are also transcriptionally active in the embryonic axes, indicating that the local catabolism of oil reserves in the embryonic axes contributes to energy production during germination. We also present evidence supporting abscisic acid inactivation and the up-regulation of gibberellin, ethylene and brassinosteroid pathways. Further, there is a remarkable differential activation of paralogous genes in these hormone signalling pathways. Taken together, our results provide insights on the regulation and biochemistry of soybean germination.
大豆（Glycine max）是全球主要的豆类作物，是蛋白质和油的重要来源。大豆基因组的发布推动了多个转录组项目，这些项目涵盖了多个发育阶段和环境条件。然而，萌发过程中胚轴的全局转录模式仍然未知。在此，我们报告了对大豆胚轴在五个萌发阶段的约15.8亿条RNA测序读数的分析。我们的结果支持了对萌发至关重要的过程的早期激活，如糖酵解、三羧酸循环和细胞壁重塑。引人注目的是，在吸水后仅3小时，蛋白激酶和转录因子就出现了优先上调，特别是来自LOB结构域家族的，这意味着转录和转录后调控在吸水后早期发挥主要作用。脂质动员和乙醛酸途径在胚轴中也具有转录活性，表明胚轴中油储备的局部分解代谢有助于萌发过程中的能量产生。我们还提供了支持脱落酸失活以及赤霉素、乙烯和油菜素内酯途径上调的证据。此外，在这些激素信号通路中，同源基因存在显著的差异激活。综上所述，我们的结果为大豆萌发的调控和生物化学提供了见解。
