Lao Shuxing, Xiong Shijiao, Fang Qi, Ye Gongyin
State Key Laboratory of Rice Biology and Breeding, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Front Physiol. 2023 Jul 14;14:1214835. doi: 10.3389/fphys.2023.1214835. eCollection 2023.
Heat shock proteins, including αB-crystallins (CRYAB), are pivotal in cellular defense mechanisms and stress response. This study presents a comprehensive investigation of heat shock proteins (HSPs), with a specific focus on the CRYAB family, within the genome of . The analysis encompasses the identification of these proteins, exploration of their phylogenetic relationships, examination of conserved domains, and evaluation of their response to high temperature conditions. A total of 46 HSPs were identified in the genome, and the differential expression of mRNA at 35°C and 25°C drew attention to five genes belonging to the CRYAB family, namely, to . The conservation level of CRYAB family genes across different species was observed to be relatively modest. Through genome-wide screening of 22 species representing six insect orders, a total of 235 CRYAB proteins were identified, with harboring eight CRYAB proteins, indicative of a moderate abundance compared to other species. Intriguingly, evolutionary analysis highlighted with potentially intricate differentiation in comparison to other members of the CRYAB family. Furthermore, RNA interference (RNAi) results demonstrated significant regulatory effects on adult lifespan under heat stress at 35°C for and . These findings lay a groundwork for future investigations into stress resistance mechanisms in parasitic wasps, providing fresh insights for the study of insect resilience amidst the backdrop of global climate change.
热休克蛋白，包括αB-晶状体蛋白（CRYAB），在细胞防御机制和应激反应中起着关键作用。本研究对热休克蛋白（HSPs）进行了全面调查，特别关注了[具体物种]基因组中的CRYAB家族。分析包括这些蛋白质的鉴定、系统发育关系的探索、保守结构域的检查以及它们对高温条件反应的评估。在[具体物种]基因组中总共鉴定出46种热休克蛋白，并且在35°C和25°C下mRNA的差异表达使人们关注到属于CRYAB家族的五个基因，即[基因名称1]到[基因名称5]。观察到CRYAB家族基因在不同物种间的保守水平相对适中。通过对代表六个昆虫目的22个物种进行全基因组筛选，总共鉴定出235种CRYAB蛋白，其中[具体物种]含有八种CRYAB蛋白，与其他物种相比显示出中等丰度。有趣的是，进化分析突出了[具体物种]与CRYAB家族其他成员相比可能存在复杂的分化。此外，RNA干扰（RNAi）结果表明，在35°C热应激条件下，[具体基因1]和[具体基因2]对成虫寿命具有显著的调节作用。这些发现为未来对寄生蜂抗逆机制的研究奠定了基础，为在全球气候变化背景下研究昆虫的适应能力提供了新的见解。
