Hall Ashley, Donohue Timothy, Peters Jason
Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, United States.
Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, Wisconsin, United States.
MicroPubl Biol. 2023 Jul 13;2023. doi: 10.17912/micropub.biology.000882. eCollection 2023.
We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replication. We provide both sequence information and plasmid maps to aid future engineering efforts.
我们展示了两种常用的接合辅助质粒pRK2013和pEVS104的完整序列。这些序列将有助于构建定制的辅助质粒,例如,带有不同抗生素标记或复制起点的质粒。我们提供了序列信息和质粒图谱,以协助未来的工程研究。