Mu Yaojia, Ma Shuai, Tan Deguan, Sun Xuepiao, Zhang Jiaming
College of Agriculture, Hainan University, Haikou, China.
Institute of Tropical Bioscience and Biotechnology, Hainan Key Laboratory of Microbiological Resources, Hainan Bioenergy Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Mitochondrial DNA B Resour. 2023 Jul 27;8(7):777-782. doi: 10.1080/23802359.2023.2237687. eCollection 2023.
Sphaeropleales is an order of fast-growing microalgae with high oil content and high efficiency in sewage treatment, in which photosynthesis plays a critical role. We isolated a strain of Sphaeropleales, ITBB-AG6 from an azolla community in a sewage pond, and sequenced its chloroplast genome. The complete genome has a length of 154 kb with a GC content of 31.7%. A total of 89 genes were annotated, including 56 protein-coding genes, 30 tRNA genes, and three rRNA genes. Out of the protein coding genes, 64.3% are involved in photosynthesis, 28.6% are involved in protein synthesis, and 7.1% are involved in ATP synthesis. Transfer RNA genes for 20 amino acids were identified, in which tRNA genes for methionine, leucine, and arginine are tripled, whereas tRNA genes for glutamic acid, glycine, serine, and threonine are doubled. Terminal inverted repeats of 27.9 kb containing 10 genes related to photosynthesis and chloroplast division are present in the genome, suggesting that photosynthesis was strengthened in the evolutionary history. Phylogenetic analysis indicates that ITBB-AG6 falls in the family Selenastraceae and is most closely related to
绿球藻目是一类生长迅速的微藻，含油量高且污水处理效率高，其中光合作用起着关键作用。我们从一个污水池塘的满江红群落中分离出一株绿球藻目菌株ITBB - AG6，并对其叶绿体基因组进行了测序。完整基因组长度为154 kb，GC含量为31.7%。共注释了89个基因，包括56个蛋白质编码基因、30个tRNA基因和3个rRNA基因。在蛋白质编码基因中，64.3%参与光合作用，28.6%参与蛋白质合成，7.1%参与ATP合成。鉴定出了20种氨基酸的转运RNA基因，其中甲硫氨酸、亮氨酸和精氨酸的tRNA基因有三倍之多，而谷氨酸、甘氨酸、丝氨酸和苏氨酸的tRNA基因有两倍之多。基因组中存在27.9 kb的末端反向重复序列，包含10个与光合作用和叶绿体分裂相关的基因，这表明在进化历史中光合作用得到了加强。系统发育分析表明，ITBB - AG6属于月牙藻科，与……关系最为密切
