胶体金免疫层析法检测油田化学品中的镉。
A colloidal gold immunoassay strip assay for cadmium detection in oilfield chemicals.
机构信息
PetroChina Research Institute of Petroleum Exploration and Development, Beijing, 10083, China.
Key Laboratory of Oilfield Chemicals, CNPC, Beijing 10083, China.
出版信息
Analyst. 2023 Aug 21;148(17):4166-4173. doi: 10.1039/d3an01075a.
Cadmium ions (Cd) are some of the major pollutants in oilfield chemicals. To reduce the pollution of oilfield chemicals, it is necessary to detect and control the content of Cd. In this study, we synthesized a highly sensitive and specific monoclonal antibody against Cd with an IC of 1.97 ng mL and no cross-reactivity. Based on this antibody, a colloidal gold immunoassay strip detection assay with an IC of 1 mg kg and a detection range of 1.0-20 mg kg in oilfield chemicals was developed. This assay could be completed in 20 min and can be used for Cd on-site testing in oilfield chemicals and improve supervision efficiency in oil exploration and development.
镉离子（Cd）是油田化学品中的主要污染物之一。为了减少油田化学品的污染，有必要检测和控制 Cd 的含量。在这项研究中，我们合成了一种针对 Cd 的高灵敏度和特异性单克隆抗体，其 IC 为 1.97ng/mL，无交叉反应。基于该抗体，开发了一种胶体金免疫层析检测试剂盒，其 IC 为 1mg/kg，检测范围为 1.0-20mg/kg，在油田化学品中的检测时间为 20 分钟，可用于油田化学品中 Cd 的现场检测，提高了石油勘探和开发过程中的监督效率。
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