胶体金免疫层析法快速检测牛奶中的锌杆菌肽。
Colloidal gold-based immunochromatographic strip assay for the rapid detection of bacitracin zinc in milk.
机构信息
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
出版信息
Food Chem. 2020 Oct 15;327:126879. doi: 10.1016/j.foodchem.2020.126879. Epub 2020 Apr 21.
In this study, a murine monoclonal antibody (mAb) of 6D2-G10 against bacitracin zinc (BAC) was produced and applied to an immunochromatographic strip (ICS) for the initial detection of BAC in milk. The ICS with a cut-off value of 25 ng/mL could be perceived by the naked eye within 10 min. With the assist of the strip reader, the limit of detection (LOD) was measured as 0.82 ng/mL, the half-maximal inhibitory concentration (IC) was recorded as 3.16 ng/mL, and the linear detection range was from 0.97 to 10.30 ng/mL. The recoveries ranged from 87.7% to 96.0% with the highest coefficient of variation (CV) of 9.1% in the intra-assay and from 84.3% to 90.2% with the highest CV of 10.7% in the inter-assay. In short, the established ICS provided a serviceable analytical tool for qualitatively and quantitatively monitoring BAC in milk.
本研究制备了一株针对杆菌肽锌（BAC）的鼠源单克隆抗体（mAb）6D2-G10，并将其应用于免疫层析条（ICS），用于初步检测牛奶中的 BAC。该 ICS 的截断值为 25ng/mL，可在 10 分钟内用肉眼观察到。借助条带阅读器，检测限（LOD）为 0.82ng/mL，半抑制浓度（IC）为 3.16ng/mL，线性检测范围为 0.97-10.30ng/mL。在内部分析中，回收率范围为 87.7%至 96.0%，最高变异系数（CV）为 9.1%，在外部分析中，回收率范围为 84.3%至 90.2%，最高 CV 为 10.7%。总之，建立的 ICS 为定性和定量监测牛奶中的 BAC 提供了一种可行的分析工具。
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