College of Pharmacy, Linyi University, Linyi 276005, P.R. China; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, P.R. China.
Linyi Academy of Agricultural Sciences, Linyi 276005, P.R. China.
Int J Med Mushrooms. 2023;25(6):41-54. doi: 10.1615/IntJMedMushrooms.2023048203.
Cordyceps militaris is a medicinal and edible mushroom. Researchers often add exogenous substances to the culture medium to increase the active substance content in C. militaris. However, the effect of earth elements on the active substance content in C. militaris and its antioxidant effects have not been reported. In this study, the active substance content in C. militaris treated with lanthanum nitrate was determined using high-performance liquid chromatography and ultraviolet spectrophotometry, and the effect on the antioxidant capacity of C. militaris after lanthanum nitrate spraying was further explored. The results showed that, in the experimental concentration range, the two concentrations of 10 mg/L and 50 mg/L had a significant influence on the active substance content of C. militaris. When the concentration of lanthanum nitrate was 10 mg/L, the synthesis of pentostatin and cordycepin was promoted. When the concentration of lanthanum nitrate was 50 mg/L, it significantly promoted the synthesis of cordycepin, and the ferric-reducing power and DPPH· scavenging rate of C. militaris treated at this concentration were significantly higher than those of the control group. However, lanthanum nitrate had no significant effect on ergosterol synthesis (P > 0.05). Finally, considering that the residual amount of lanthanum in C. militaris and the residual amount of lanthanum in 50 mg/L lanthanum nitrate-treated C. militaris is within the allowable daily intake of 4.2 mg for humans, the optimal concentration of lanthanum nitrate-treated C. militaris is 50 mg/L.
蛹虫草是一种药食两用的真菌。研究人员常向培养基中添加外源物质来提高蛹虫草中活性物质的含量。然而，尚未有关于土壤元素对蛹虫草中活性物质含量的影响及其抗氧化作用的报道。本研究采用高效液相色谱法和紫外分光光度法测定了硝酸镧处理蛹虫草后的活性物质含量，并进一步探讨了硝酸镧喷雾处理对蛹虫草抗氧化能力的影响。结果表明，在实验浓度范围内，10mg/L 和 50mg/L 这两个浓度对蛹虫草活性物质含量有显著影响。当硝酸镧浓度为 10mg/L 时，促进了蛹虫草中麦角甾醇和蛹虫草素的合成；当硝酸镧浓度为 50mg/L 时，显著促进了蛹虫草素的合成，且该浓度处理的蛹虫草的铁还原力和 DPPH·清除率均显著高于对照组。然而，硝酸镧对麦角固醇的合成没有显著影响（P＞0.05）。最后，考虑到蛹虫草中残留的镧量和 50mg/L 硝酸镧处理的蛹虫草中残留的镧量均在人体每日允许摄入量 4.2mg 以内，因此，硝酸镧处理蛹虫草的最佳浓度为 50mg/L。
