Bureau Jean-Alexandre, Oliva Magdalena Escobar, Dong Yueming, Ignea Codruta
Department of Bioengineering, McGill University, Montreal, QC, H3A 0C3, Canada.
Nat Prod Rep. 2023 Dec 13;40(12):1822-1848. doi: 10.1039/d3np00005b.
Covering: 2011-2022The low amounts of terpenoids produced in plants and the difficulty in synthesizing these complex structures have stimulated the production of terpenoid compounds in microbial hosts by metabolic engineering and synthetic biology approaches. Advances in engineering yeast for terpenoid production will be covered in this review focusing on four directions: (1) manipulation of host metabolism, (2) rewiring and reconstructing metabolic pathways, (3) engineering the catalytic activity, substrate selectivity and product specificity of biosynthetic enzymes, and (4) localizing terpenoid production enzymatic fusions and scaffolds, or subcellular compartmentalization.
2011年至2022年
植物中萜类化合物产量较低以及合成这些复杂结构存在困难，这促使人们通过代谢工程和合成生物学方法在微生物宿主中生产萜类化合物。本综述将涵盖利用工程酵母生产萜类化合物的进展，重点关注四个方向：（1）宿主代谢的调控；（2）代谢途径的重新布线和重建；（3）生物合成酶的催化活性、底物选择性和产物特异性的工程改造；（4）通过酶融合和支架或亚细胞区室化实现萜类化合物生产的定位。
