位点特异性重组中的链交换。
Strand exchange in site-specific recombination.
作者信息
Enquist L W, Nash H, Weisberg R A
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1363-7. doi: 10.1073/pnas.76.3.1363.
Abstract
The site-specific recombination system of phage lambda promotes crossovers at its attachment site (att). In this report we show that when phage are crossed in conditions where only the site-specific recombination system is active, a low frequency of crossovers can also be detected in a region that is close to but does not contain att. These crossovers require the phage int gene, the host hip gene, and the integrity of att. They are not detected if one of the parents carries a substitution of a heterologous attachment site (attB instead of attP). To explain these findings we suggest that site-specific recombination can proceed by exchange of single strands between the participating chromosomes at att and migration of the resulting junction outside of att.
摘要
噬菌体λ的位点特异性重组系统促进其附着位点（att）处的交叉。在本报告中，我们表明，当噬菌体在仅位点特异性重组系统活跃的条件下进行杂交时，在靠近但不包含att的区域也能检测到低频交叉。这些交叉需要噬菌体int基因、宿主hip基因以及att的完整性。如果亲本之一携带异源附着位点（attB而非attP）的替代，则无法检测到这些交叉。为了解释这些发现，我们提出位点特异性重组可以通过参与染色体在att处单链交换以及由此产生的连接点在att之外迁移来进行。
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