Key Laboratory of Vegetation Ecology of the Ministry of Education, Institute of Grassland Science, Northeast Normal University, Changchun, China.
Tonghua Normal University, Tonghua, China.
Sci Rep. 2023 Jul 31;13(1):12345. doi: 10.1038/s41598-023-39501-y.
Phosphorus (Pi) deficiency is a major factor of limiting plant growth. Using Phosphate-solubilizing microorganism (PSM) in synergy with plant root system which supply soluble Pi to plants is an environmentally friendly and efficient way to utilize Pi. Trichoderma viride (T. viride) is a biocontrol agent which able to solubilize soil nutrients, but little is known about its Pi solubilizing properties. The study used T. viride to inoculate Melilotus officinalis (M. officinalis) under different Pi levels and in order to investigate the effect on Pi absorption and growth of seedlings. The results found that T. viride could not only solubilizate insoluble inorganic Pi but also mineralize insoluble organic Pi. In addition, the ability of mineralization to insoluble organic Pi is more stronger. Under different Pi levels, inoculation of T. viride showed that promoted the growth of aboveground parts of seedlings and regulated the morphology of roots, thus increasing the dry weight of seedlings. The effect of T. viride on seedling growth was also reflected the increasing of chlorophyll fluorescence parameters and photosynthetic pigment content. Moreover, compared to the uninoculated treatments, inoculation of T. viride also enhanced Pi content in seedlings. Thus, the T. viride was a beneficial fungus for synergistic the plant Pi uptake and growth.
磷（Pi）缺乏是限制植物生长的主要因素。将解磷微生物（PSM）与植物根系协同作用，为植物提供可溶性 Pi，是一种环保且高效的利用 Pi 的方法。木霉（T. viride）是一种能够溶解土壤养分的生物防治剂，但人们对其解磷特性知之甚少。本研究使用 T. viride 接种草木樨（M. officinalis），在不同 Pi 水平下，以研究其对幼苗 Pi 吸收和生长的影响。结果发现，T. viride 不仅可以溶解不溶性无机 Pi，还可以矿化不溶性有机 Pi。此外，矿化不溶性有机 Pi 的能力更强。在不同 Pi 水平下，接种 T. viride 可促进幼苗地上部分的生长，调节根系形态，从而增加幼苗的干重。T. viride 对幼苗生长的影响还体现在增加叶绿素荧光参数和光合色素含量上。此外，与未接种处理相比，接种 T. viride 还可提高幼苗中的 Pi 含量。因此，T. viride 是一种有益于植物 Pi 吸收和生长的有益真菌。
