Jan Ishrat, Dar Alamgir A, Mukhtar Malik, Shah Mehraj D, Wani Ashraf A, Dar Shahnawaz R, Dar Irshad H, Sofi Javid A
Research Centre for Residue and Quality Analysis, Sher-e-Kashmir University of Agricultural Sciences and Technology (SKUAST-K), Srinagar, India.
Biomed Chromatogr. 2023 Oct;37(10):e5705. doi: 10.1002/bmc.5705. Epub 2023 Jul 31.
The present study on "acephate persistence on green pea" was conducted in SKUAST-Kashmir. The study aimed to determine the persistence, dissipation kinetics and waiting period of acephate on green pea. Acephate was sprayed at 75% soluble powder (SP) at 560 g a.i.ha at the fruiting stage followed by another application at a 10 day interval. A rapid and accurate method (quick, easy, cheap, effective, rugged and safe, QuEChERS) was used for extraction and the residue was determined by gas chromatography-electron capture detection on a CPSIL-8CB capillary column (0.25um film thickness, 0.25 mm i.d, 30 m length). At the fortification levels of 0.05, 0.1 and 0.5 mg kg , the percentage recovery of acephate on green pea was found in the range of 71-107%. The initial deposit of green pea was estimated to be 0.37 mg kg . At the indicated dose, the residue of acephate on green pea dissipated below the limit of quantification of 0.05 mg kg after 10 days. Acephate degradation was quick in green pea, with a half-life of 4.07 days. For safe eating of green peas, a 10 day waiting period is recommended. The gas chromatography-electron capture detection technique was validated by following the SANTE standards.
本关于“乙酰甲胺磷在青豆上的残留情况”的研究在克什米尔农业科学技术大学（SKUAST-Kashmir）开展。该研究旨在测定乙酰甲胺磷在青豆上的残留情况、消解动力学及安全间隔期。在结果期，以75%可溶性粉剂（SP）的形式，按560克有效成分/公顷的用量喷施乙酰甲胺磷，之后每隔10天再喷施一次。采用一种快速准确的方法（快速、简便、廉价、高效、耐用且安全的QuEChERS方法）进行提取，并通过气相色谱 - 电子捕获检测法在CPSIL - 8CB毛细管柱（膜厚0.25微米、内径0.25毫米、长度30米）上测定残留量。在0.05、0.1和0.5毫克/千克的加标水平下，发现乙酰甲胺磷在青豆上的回收率在71%至107%之间。青豆上的初始沉积量估计为0.37毫克/千克。在指定剂量下，10天后乙酰甲胺磷在青豆上的残留量降至0.05毫克/千克的定量限以下。乙酰甲胺磷在青豆中的降解很快，半衰期为4.07天。为了安全食用青豆，建议设定10天的安全间隔期。气相色谱 - 电子捕获检测技术按照SANTE标准进行了验证。
