Research Centre for Residue and Quality Analysis, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, J&K, 190025, India.
Biomed Chromatogr. 2022 Jul;36(7):e5373. doi: 10.1002/bmc.5373. Epub 2022 Mar 30.
The present work describes the persistence, dissipation behaviour, half-life, risk assessment and novel gas chromatography method for the residue estimation of cypermethrin in green pea by spraying cypermethrin 10EC at 50 g a.i. ha at fruiting stage followed by another application at a 10 day interval. The sample extraction and cleanup was followed bya modified quick, easy, cheap, effective, rugged, and safe method, and the residues of cypermethrin were determined using a validated gas chromatography method. The initial deposits were found to be 1.21 mg kg following the application of insecticide at 50 g a.i. ha . Cypermethrin residues declined to below the detection limit of 0.05 mg kg after 15 days at the recommended dosage. The half-life of cypermethrin was 2.66 days at 50 g a.i. ha . For risk assessment studies, the waiting period of 15 days is recommended as safe for consumption for the insecticide. The GC-ECD method was validated according to the SANTE guidelines by various analytical parameters including linearity, accuracy, detection and quantification limits. The developed method is simple, selective and repeatable, and can be used for the standardization of pesticides on fruits and vegetables.
本工作描述了氯氟氰菊酯在豌豆中的持留、消解行为、半衰期、风险评估和残留量的气相色谱分析方法。在果实期以 50 g a.i. ha 的剂量喷雾施用氯氟氰菊酯 10EC 后，间隔 10 天再进行另一次施药。采用改良的快速、简便、廉价、有效、耐用和安全方法进行样品提取和净化，并用验证的气相色谱法测定氯氟氰菊酯的残留量。施药 50 g a.i. ha 后，初始沉积量为 1.21 mg kg。在推荐剂量下，15 天后，氯氟氰菊酯残留量下降至低于检测限 0.05 mg kg。氯氟氰菊酯在 50 g a.i. ha 的半衰期为 2.66 天。为了进行风险评估研究，建议在推荐剂量下等待 15 天，以确保该杀虫剂食用安全。根据 SANTE 指南，采用线性、准确度、检测限和定量限等各种分析参数对 GC-ECD 方法进行了验证。所建立的方法简单、选择性好、重复性高，可用于水果和蔬菜上农药的标准化。
