Yiğit Serbulent, Kul Selim, Aci Recai, Keskin Adem, Tuygun Tuğçe, Duman Esra
Department of Genetics, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkiye.
Department of Medical Biology, Graduate Institute, Ondokuz Mayis University, Samsun, Turkiye.
Mol Biol Res Commun. 2023;12(3):109-115. doi: 10.22099/mbrc.2023.47336.1827.
In this study, the relationship between 23bp indel genotype and allele frequency with twin pregnancy, fertility, live weight and milk yield in 106 female Akkaraman ewes raised in Elazığ province was investigated. In the study conducted in Elâzığ province, 10ml milk was collected from 106 Akkaraman sheep and DNA was extracted from these milk. In 23bp indel genotype frequency, genotype was found more than and genotypes and 23bp indel ın allele frequency, the allele was found to be higher than the I allele. In both the first and second parity, the twinning rate was found to be lower. In both the first and second parity, the twinning rate was higher in the genotype, and it was observed that this genotype promınated mıddle lıvestock weıght and mılk yıeld. According to the results of our study, mutations in the gene, which is a gene affecting reproductive efficiency in sheep, do not have a positive effect on fertility and twinning rate in Akkaraman sheep. To sum, this study provided theoretical references for the comprehensively research of the function of gene and the breeding of Akkaraman Sheep. The 23-bp indel variants can be considered as molecular markers for litter size of sheep for marker-assisted selection breeding.
在本研究中，调查了在埃尔津詹省饲养的106只雌性阿克卡拉曼母羊中23bp插入缺失基因型和等位基因频率与双胎妊娠、繁殖力、活体重和产奶量之间的关系。在埃尔津詹省进行的研究中，从106只阿克卡拉曼羊中采集了10毫升牛奶，并从这些牛奶中提取了DNA。在23bp插入缺失基因型频率方面，发现基因型多于基因型和基因型，在23bp插入缺失等位基因频率方面，发现等位基因高于I等位基因。在第一胎和第二胎中，双胎率均较低。在第一胎和第二胎中，基因型的双胎率较高，并且观察到该基因型在中等家畜体重和产奶量方面占主导地位。根据我们的研究结果，绵羊中影响繁殖效率的基因中的突变对阿克卡拉曼羊的繁殖力和双胎率没有积极影响。总之，本研究为全面研究基因的功能和阿克卡拉曼羊的育种提供了理论参考。23bp插入缺失变异可作为绵羊产仔数的分子标记用于标记辅助选择育种。
